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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 19 (1988), S. 63-71 
    ISSN: 1573-5079
    Keywords: CO2 fixation ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract From studies conducted in both our laboratory and by Gibson, Tabita and colleagues, as well as drawing on the recent studies with Alcaligenes eutrophus, we describe two genetic regions which have been identified on the chromosome of Rhodobacter sphaeroides which code for a number of enzymes involved in CO2 fixation. One region was found to contain the genes coding for fructose 1,6-bisphosphatase (fbpA), phosphoribulokinase (prkA), a 37 kDa polypeptide (cfxA), and form I ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL, S). These genes appear to be expressed in the same transcriptional direction and are tandomly arranged. A second, apparently unlinked region of the chromosome contains a duplicate (with respect to functionality of gene products) but not identical set of these same four genes. Although the gene order in both regions is apparently identical, there is approximately 4 kb of DNA separating the 3′-end of prkB and the beginning of cfxB. The specific genetic organizations and proposed roles of these two genetic regions are discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 19 (1988), S. 39-61 
    ISSN: 1573-5079
    Keywords: Bacteriochlorophyll-protein complex ; gene expression ; light-harvesting complex ; reaction center ; Rhodospirillaceae ; transcriptional control ; translational control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The puf operon of the purple nonsulfur photosynthetic bacterium, Rhodobacter sphaeroides, contains structural gene information for at least two functionally distinct bacteriochlorophyll-protein complexes (light harvesting and reaction center) which are present in a fixed ratio within the photosynthetic intracytoplasmic membrane. Two proximal genes (pufBA) specify subunits of a long wavelength absorbing (i.e., 875 nm) light harvesting complex which are present in the photosynthetic membrane in ≃15 fold excess relative to the reaction center subunits which are encoded by the pufLM genes. This review summarizes recent studies aimed at determining how expression of the R. sphaeroides puf operon region relates to the ratio of individual bacteriochlorophyll-protein complexes found within the photosynthetic membrane. These experiments indicate that puf operon expression may be regulated at the transcriptional, post-transcriptional, translation and post-translational levels. In addition, this review discusses the possible role(s) of newly identified loci upstream of pufB which may be involved in regulating either synthesis or assembly of individual bacteriochrlorophyll-protein complexes as well as the pufX gene, the most distal genetic element within the puf operon whose function is still unknown.
    Type of Medium: Electronic Resource
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