ISSN:
0730-2312
Keywords:
estradiol-dependent trans-acting factor
;
binds preferentially
;
dyad-symmetry structure
;
third intron
;
avian vitellogenin gene
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
The secondary activation of the avian vitellogenin II gene in isolated liver nuclei by cytoplasmatic liver extracts of estradiol-treated chicks is accompanied by the binding of a protein from the extract to the structural part of the cloned gene. Both the DNA-binding and gene-stimulatory activities, which cochromatograph on heparin-Sepharose, are apparently present only in the cytoplasmatic liver extracts of estradiol-treated roosters and in the oviduct extracts of egg-laying hens. DNA-binding competition assays combined with exonuclease III footprinting showed that the factor binds to the imperfect dyad-symmetry structure 5‘GTCTTGTTCCAAAC3’ within the third intron of the gene. The factor is sequence specific and binds equally well to both single- and double-stranded DNA with an estimated dissociation constant of 3.5 × 10-10M.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcb.240350106
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