ISSN:
1573-5028
Keywords:
L-asparaginase
;
DNA sequence
;
developing seed
;
Lupinus angustifolius (lupin)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5′ flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genomic sequence with that of a L. arboreus cDNA. Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5′ of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5′ flanking region contained sequences associated with seed-specific expression.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00014503
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