ISSN:
1573-4986
Keywords:
ceramide glycanase
;
4-methylumbelliferyl 6′-O-benzyl-β-d-lactoside derivative
;
fluorometric assay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract 4-Methylumbelliferyl 6′-O-benzyl-β-d-lactoside (6′Bn-MU-Lac) and some related compounds were synthesizedvia different selective reactions including phase-transfer glycosylation. Their suitability as substrates for a fluorometric assay of ceramide glycanase (CGase) was evaluated. Among others, the 6′Bn-MU-Lac, which is resistant to exogalactosidase, was found to be a suitable substrate for routine assay of the CGase activity. For American leech CGase, theK m value is 0.232 mM at pH 5.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00731468
Permalink