ISSN:
1573-2592
Keywords:
Thyroid-stimulating hormone
;
human lymphocytes
;
biotinylation
;
fluorescein-activated cell sorting
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The expression and functional relevance of thyroid stimulating hormone (TSH) receptors on human immune cells were studied. Flow cytometric analysis was used to study the binding of biotinylated TSH to human peripheral blood mononuclear cells (PBMC) and various purified lymphoid populations. Our results indicate that the hormone binds well to monocytes and natural killer (NK) cells and marginally to purified tonsillar T and B lymphocytes. There was a significant increase in the binding of TSH to purified B cells that were activatedin vitro withStaphylococcus aureaus Cowan. In contrast, the binding of TSH to T cells was unaltered when they were stimulated with phytohemagglutinin (PHA). While TSH increases DNA synthesis and intracellular cAMP levels of FRTL-5 rat thyroid cells, it did not have such stimulatory effects on lymphocytes. However, there was a moderate increase in Ig production by activated B lymphocytes when they were cultured in the presence of the hormone. A possible function for TSH as a link between the immune system and the thyroid is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00918653
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