ISSN:
1432-0983
Keywords:
Zygote-specific genes
;
Multigene family
;
Cysteine-rich
;
Glutamine-rich
;
Chlamydomonas reinhardtii
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have obtained five cDNA clones which are complementary to transcripts that accumulate specifically during the early stage of zygote formation in Chlamydomonas reinhardtii. Based on the time-course of transcript accumulation of their corresponding genes, these clones were classified into two groups: an early expression group (three clones; pZS102-1, pZS102-69, pZS1802) and a late expression group (two clones; pZS1860 and pZS1886). The levels of transcripts in the former group reached a maximum 30 min after the onset of mating, whereas those in the latter group peaked at 3 h. The addition of 10 μg/ml of cycloheximide reduced the decline in the levels of transcripts, except in the transcript corresponding to pZS102-69. Two clones, pZS102-1 and pZS102-69, contained highly-homologous predicted open-reading frames, and were judged to be cDNAs for two members of a novel gene family. These genes were referred to as zys1A and zys1B, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00336779
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