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  • 1
    Electronic Resource
    Electronic Resource
    Temperature and the energy cost of oscillatory work in teleost fast muscle fibres (1991)
    Springer
    Pflügers Archiv 419 (1991), S. 177-183 
    Details
    ISSN: 1432-2013
    Keywords: Temperature ; Fish muscle ; Oscillatory work ; Energetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Bundles of 20–30 fast muscle fibres were isolated from the abdominal myotomes of the short-horned sculpin (Myoxocephalus scorpius L.). The energy cost of contraction was measured during oscillatory work at 4 °C and 15 °C following treatment with iodoacetate and nitrogen gas to block glycolysis and aerobic metabolism. Isolated fibres were subjected to sinusoidal length changes about in situ resting length and stimulated at a selected phase in the strain cycle. Preliminary experiments with untreated preparations established the strain amplitude and stimulation parameters required to maximize work output over a range of cycle frequencies at 4 °C and 15 °C. Following oscillatory work, treated preparations were rapidly frozen, freeze-dried and the concentrations of phosphocreatine (PCr), creatine, adenosine 5′-triphosphate (ATP), adenosine 5′- di- and mono-phosphate and inosine 5-monophosphate measured by high performance liquid chromatography. The concentration of PCr declined in proportion to the total work done for up to 64 cycles without a significant change in ATP. Maximum power output was produced at a cycle frequency of 5 Hz at 4 °C (14–18 W/kg) and 17 Hz at 15 °C (23–27 W/kg). The rate of utilization of PCr per cycle was independent of temperature. However, since work per cycle was higher at 4 °C (2.7–3.7 mJ/g wet weight) than 15 °C (1.2–1.6 mJ/g wet weight), the energetic cost of contraction decreased with increasing temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00373004
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  • 2
    Electronic Resource
    Electronic Resource
    Evolutionary adaptation of muscle power output to environmental temperature: force-velocity characteristics of skinned fibres isolated from antarctic, temperate and tropical marine fish (1985)
    Springer
    Pflügers Archiv 405 (1985), S. 136-140 
    Details
    ISSN: 1432-2013
    Keywords: Muscle ; Temperature ; Mechanics ; Forcevelocity ; Skinned fibres ; Antarctic fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract 1. Single fast fibres were isolated from the myotomal muscles of icefish (Chaenocephalus aceratus Lönnberg, Antarctica), North Sea Cod (Gadus morhua L.) and Pacific Blue Marlin (Makaira nigricans Wakiya, Hawaii). Fibres were chemically skinned with the non-ionic detergent Brij-58. 2. Maximum tensions (Po, kN m−2) developed at the characteristic body temperature of each species are 231 for icefish (−1°C), 187 for cod (8°C) and 156 for marlin (20°C). At 0°CPo is 7 times higher for fibres from the icefish than from the marlin. 3. Fibres from icefish and cod failed to relax completely following activations at temperatures above approximately 12°C. The resultant post-contraction force is associated with a proportional increase in stiffness, suggesting the formation of a population of Ca-insensitive cross bridges. 4. At 0°C there is little interspecific variation in unloaded contraction velocity (V max) among the three species.V max (muscle lengths s−1) at normal body temperatures are 0.9 for icefish (−1°C), 1.0 for cod (8°C) and 3.4 for marlin (20°C). 5. The force-velocity (P-V) relationship becomes progressively more curved with increasing temperature for all three species. 6. Maximum power output for the fast muscle fibres from the Antarctic species at −1°C is around 60% of that of the tropical fish at 20°C. Evolutionary temperature compensation of muscle power output appears largely to involve differences in the ability of cross bridges to generate force.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584534
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