ISSN:
1573-2592
Keywords:
Protein kinase C isozymes
;
T cell activation
;
B cell activation
;
interleukin-2
;
interleukin-2 receptors
;
CD4+ T cells
;
CD8+ T cells
;
phorbol esters
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract To determine a role of protein kinase C (PKC) isozymes in lymphocyte activation, human peripheral blood mononuclear cells were activated with 12-deoxyphorbol-13-O-phenylacetate (dPP; an agonist of both calcium-dependent and calcium-independent PKC isozymes), thymeleatoxin (TX; an activator of calcium-dependent PKCα, β, and γ), and 12-deoxyphorbol-13-O-phenylacetate 20 acetate (dPPA; an activator of PKCβ1 isozyme) and examined for DNA synthesis, lymphocyte proliferation, interleukin-2 (IL-2) production, expression of IL-2 receptor α and β chains on CD3+, CD4+, and CD8+ T lymphocytes and CD20+ B lymphocytes, and translocation of PKCβ isozyme from cytosol to membrane fraction. The results show that dPPA activates lymphocytes by inducing the above changes in a manner analogous to that of dPP, TX, and phorbol myristate acetate. These data suggest that PKCβ1 is involved in the activation of human peripheral blood T and B lymphocytes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01552311
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