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  • 1
    Electronic Resource
    Electronic Resource
    Nucleotide base sequence of the mitochondrial COIII gene of Schizophyllum commune (1988)
    Springer
    Current genetics 14 (1988), S. 401-403 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; COIII ; mtDNA ; Basidiomycete
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cytochrome oxidase subunit III gene (COIII) of the Badidiomycete S. commune has been identified, cloned, and sequenced. The gene contains no introns, is AT-rich (69%) and exhibits a high degree of similarity to sequences from Ascomycetes. While most mitochondrial genes use both TGA and TGG to specify tryptophan, the COIII gene of Schizophyllum uses TGG exclusively. Translation requires no deviation from the universal code.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419999
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  • 2
    Electronic Resource
    Electronic Resource
    The isolation of specific genes from the basidiomycete Schizophyllum commune (1987)
    Springer
    Current genetics 12 (1987), S. 547-554 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; Transformation ; Gene isolation ; Basidiomycetes ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have developed a routine way to isolate genes directly from the basidiomycete fungus, Schizophyllum commune. Plasmid DNA from a genomic gene library was used to isolate five specific genes by complementation of Schizophyllum mutations via transformation. The mutant strains were deficient in the ability to synthesize either adenine (ade2 and ade5), uracil (ural, encoding orotidine-5′-phosphate decarboxylase; OMPdecase), tryptophan (rpl, encoding indole-3-glycerol phosphate synthetase; IGPS) or para aminobenzoic acid (pab1). In each case, Southern analysis revealed that transformation to prototrophy was concomitant with the integration of vector sequence into the genome of the S. commune mutant. Total DNA from transformants was restricted, religated, and used to transform E. coli. Ampicillin resistant plasmids were recovered from E. coli and tested for their ability to transform the corresponding mutant of S. commune. Plasmids complementing the ade2, adeS, pabl, trpl, and ural mutations were recovered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419565
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning and characterization of aSchizophyllum gene withAβ6 mating-type activity (1996)
    Springer
    Current genetics 29 (1996), S. 136-142 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; Mating type ; Homeodomain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The A-pathway of development in the basidiomycete fungusSchizophyllum commune may be activated by either of two mating-type loci,Aξ andAβ Aα consists of two multiallelic genes,Y andZ. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non- self combinations of Y and Z form heteromultimers which are thought to be transcription factors of developmental genes. To more completely understand A-regulated development it is necessary to address the issue of functional redundancy, i.e., how do two different mating loci,Aα andAβ, both manage to regulate the same pathway. Here we report the structure of a gene withAβ6 activity. This gene, denotedAβV6, encodes a deduced polypeptide of 640 amino-acids with a homeodomain motif. V6 also contains a 20-amino acid sequence that is conserved in Aα Y1, Y3 and Y4. Except for the homeodomain and the conserved sequence, the deduced V6 polypeptide shows no significant identity to AαY, AαZ, or other known proteins. The presence of a homeodomain suggests that V, like Y and Z, may be a regulatory protein for genes in the A-pathway. Thus whileAα andAβ encode different proteins, the general mechanism by which Aα and Aβ components signal A-regulated development may be similar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221577
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  • 4
    Electronic Resource
    Electronic Resource
    The Aα mating-type locus ofSchizophyflum commune: Structure and function of geneX (1996)
    Springer
    Current genetics 29 (1996), S. 143-149 
    Details
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; Mating type ; Gene replacement ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract GeneX maps immediately right of geneY at the Aα mating-type locus inSchizophyllum commune. AllelesX1, X3 andX4 were isolated, subcloned, and sequenced. The structure of the alleles and their relationship to the Aα locus is described. The deducedX isoforms possess no recognized motifs common to other polypeptides and no significant similarity to any sequences in the protein databases.X alleles do not activate A-regulated development when transformed into recipient strains possessing different Aα mating types or when these transformants are mated with tester strains. AnX1-disrupted construction yielded a high frequency (33%) of homologous gene replacements.X-disrupted mutants have wild-type phenotypes and mate normally. Both the functional analyses and sequence data forX1,X3, andX4 suggest that the right boundary of the Aα mating-type locus falls betweenAαY andX. We propose that theZ andY genes constitute the Aoc locus in its entirety.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221578
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  • 5
    Electronic Resource
    Electronic Resource
    Cloning and comparison of Aα mating-type alleles of the basidiomycete Schizophyllum commune (1989)
    Springer
    Molecular genetics and genomics 218 (1989), S. 72-77 
    Details
    ISSN: 1617-4623
    Keywords: Schizophyllum commune ; Mating type ; Incompatibility ; Multiallelic ; Self/nonself recognition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An Aα mating-type allele (Aα4) was isolated by walking the chromosome from the closely linked PAB1 gene. A cosmid clone containing the Aα1 allele isolated from the walk was used as a probe to recover the Aα1 allele from another cosmid library. Cosmids encoding mating-type activity were identified by transforming Schizophyllum cells and screening for activation of A-regulated development. Putative mating-type transformants were confirmed in mating tests and genetic analyses of progeny. The identity of the specific alleles isolated was demonstrated by showing that their effectiveness in transforming for mating type is limited to recipient strains possessing an Aα allele different from the one encoded by the cloned sequences. Transforming DNA is active in trans, suggesting that Aα encodes a diffusible product. Restriction mapping shows that Aα1 and Aα4 are coded in the same physical region of the genome, but within a subregion that contains extensive sequence divergence. In addition, Southern analyses show that there is only one copy of Aα1 or Aα4 per haploid genome, and that they do not cross-hybridize to one another or to any of the other Aα alleles. Aα1 and Aα4 were subcloned as 2.8 and 1.2 kb fragments, respectively, retaining in transformation all the mating-type activity demonstrated of the original cosmids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330567
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  • 6
    Electronic Resource
    Electronic Resource
    Only one of the paired Schizophyllum commune Aα mating-type, putative homeobox genes encodes a homeodomain essential for Aα-regulated development (1994)
    Springer
    Molecular genetics and genomics 244 (1994), S. 318-324 
    Details
    ISSN: 1617-4623
    Keywords: Schizophyllum commune ; Homeodomain ; Homeobox ; Mating type
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Aα mating-type locus is one of four master regulatory loci controlling sexual development in Schizophyllum commune. The Aα locus contains two homeobox genes, Y and Z, encoding two homeodomain-related proteins, Y and Z, Y and Z are each multi-allelic genes. When haploid strains form fusion cells, only particular combinations of Y and Z alleles activate Aα-regulated sexual development. The role of the putative homeodomain was examined in several Y and Z alleles by site-directed mutagenesis of regions critical to secondary structure and function of homeodomains. Mutations of the Z homeobox do not affect the function of Z proteins in Aα-activated development, but mutations of Yhomeoboxes destroy the ability of Y proteins to activate development. We conclude that only one of two Aα homeodomain-related regulators relies upon the homeodomain motif to effect gene expression in sexual development. This conclusion affords a refinement of our working hypothesis for the mechanism by which Aα proteins may regulate target gene expression. On the basis of our results with the Z protein, we speculate that the DNA-binding motifs of some transcriptional regulators may be lost or modified during evolution once these regulators have been recruited to participate in complexes with other DNA-binding proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285460
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