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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Gene 87 (1990), S. 177-183 
    ISSN: 0378-1119
    Keywords: Pathogenic fungi ; Saccharomyces cerevisiae ; antifungals ; erg7 mutant ; ergosterol biosynthesis ; plasmids ; recombinant DNA
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of aquatic ecosystem stress and recovery 1 (1992), S. 283-293 
    ISSN: 1573-5141
    Keywords: fish population ; growth ; reproduction ; assessment ; health
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Since there are not any well developed procedures for site-specifically evaluating the health of fish populations, most field surveillance programs have been restricted to collecting information on chemistry, toxicity, bioaccumulation, biochemical alterations orin situ benthic community structure. Identification of the mechanism and significance of contaminant effects on fish populations depends on the ability to identify changes and to distinguish changes in survival, food availability or food conversion efficiency, from coincidental changes associated with alterations in habitat or natural variability. Preliminary identification of the characteristics of adult fish can be used to cost-effectively focus financial resources on the alterations which are of relevance to interpretation of impacts and identification of causal factors. Proper interpretation requires that appropriate attention be given to monitoring level and strategy, selection of species and timing of sampling, sample size requirements and choice of reference site.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of aquatic ecosystem stress and recovery 3 (1994), S. 227-237 
    ISSN: 1573-5141
    Keywords: fish population ; assessment ; monitoring ; framework ; tiered-testing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We previously proposed a classification scheme to describe the responses of fish populations to anthropogenic stress. The original framework separated impacted populations into five response patterns, but had several limitations and deficiencies, including the subsequent description of additional patterns and the absence a pattern reflecting no detectable response of the population. The response framework has been reorganized based on new information and response patterns described in published literature. The sentinel monitoring framework provides a tool for initial interpretation of adult fish surveys and description of priority areas for subsequent studies to allow identification of the mechanism of impact. Subsequent, focused, follow-up studies are required to identify the stressor(s) once the impact mechanism has been identified. Response pattern separation requires information from the sampled fish population including age structure, energy expenditure and energy storage. The patterns represent characteristic responses to changes in food availability, adult mortality, recruitment failure and metabolic disruption. The framework requires the identification and appropriate sampling of comparable reference sites, but the sentinel monitoring framework has proved to be a useful tool in preliminary trials, and should provide a focal point for cumulative effects studies using fish populations.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 14 (1998), S. 565-571 
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; electroporation ; transformation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-fold improvement in transformation frequency was achieved with several mutant strains of S. cerevisiae that transformed poorly by conventional procedures. Both DTT and lithium acetate were necessary for maximal transformation frequencies. Pretreatment with lithium and DTT also resulted in an ∼3·5-fold increase in the electroporation transformation frequency of the pathogenic fungus Candida albicans. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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