ISSN:
1573-4927
Keywords:
SODIUM-CALCIUM EXCHANGER HEART BALB
;
C MOUSE C57BL
;
MOUSE CLONING
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A truncated isoform of the cardiacsodium-calcium exchanger (NCX1) was identified andcloned from BALB/c mouse heart. This cDNA clone has anAATAAA polyadenylation signal in the 3′untranslated region that caused the 3344-bp clone to stop at a prematuretermination site and encode for a 940-amino acid (AA)protein, in contrast to the wild-type C57BL/6 mouse,which has a 970-AA protein. Comparing the predicted AA sequence of NCX1 between the two mousestrains by hydropathic plot, we found that the BALB/cNCX1 protein has only 11 extracellular domains, missingone domain at the COOH terminal, while C57BL/6 mice have 12 extracellular domains, similar to otherspecies. Using the mouse mapping gene panel BCB, theNCX1 gene was mapped to the distal end of mousechromosome 17 (51.3 cM), confirming the data published from the rat probe.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018760421627
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