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  • Rhizobium trifolii  (1)
  • Tannin  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Organisation of nodulation and nitrogen fixation genes on a Rhizobium trifolii symbiotic plasmid (1984)
    Springer
    Archives of microbiology 139 (1984), S. 151-157 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium trifolii ; Symbiosis ; Nodulation ; Nitrogen fixation ; Symbiotic genes ; Reiterated sequences ; Plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Rhizobium trifolii symbiotic plasmid specific gene library was constructed and the physical organisation of regions homologous to nifHDK, nifA and nod genes was determined. These symbiotic gene regions were localised to u 25 kb region on the sym-plasmid, pPN1. In addition four copies of a reiterated sequence were identified on this plasmid, with one copy adjacent to nifH. No rearrangement of these reiterated sequences was observed between R. trifolii bacterial and bacteroid DNA. Analysis of a deletion derivative of pPN1 showed that these sequences were spread over a 110 kb region to the left of nifA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401991
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The requirement for exopolysaccharide precedes the requirement for flavolan-binding polysaccharide in nodulation of Leucaena leucocephala by Rhizobium loti (1997)
    Springer
    Archives of microbiology 167 (1997), S. 182-186 
    Details
    ISSN: 1432-072X
    Keywords: Key words Exopolysaccharide ; Invasion ; Tannin ; Indeterminate nodules ; Lotus ; Leucaena ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhizobium loti strain PN4115 (NZP2213 str-1) ineffectively nodulates Leucaena leucocephala, i.e., strain PN4115 induces nodulation (Nod+) and is able to invade these nodules (Inv+), but fails to fix nitrogen (Fix–). Strain PN4115 does not synthesize a flavolan-binding polysaccharide (FBP), which is synthesized by the fully effective (Nod+Inv+Fix+) R. loti strain PN184 (NZP2037 str-1). The FBP may offer protection from prodelphinidin-rich flavolans synthesized by Lc. leucocephala. In this work, we show that exopolysaccharide (EPS)-negative mutants derived from strain PN4115 have a more severe ineffective phenotype (Nod+Inv–Fix–) on Lc. leucocephala than strain PN4115. This suggests that EPS from strain PN4115 is functional during invasion of Lc. leucocephala and that the requirement for EPS precedes the requirement for FBP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050433
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    Paper (German National Licenses)
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