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  • pSR1 plasmid  (4)
  • Regulatory circuit  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Mode of expression of the positive regulatory genes PHO2 and PHO4 of the phosphatase regulon in Saccharomyces cerevisiae (1989)
    Springer
    Molecular genetics and genomics 217 (1989), S. 31-39 
    Details
    ISSN: 1617-4623
    Keywords: Autoregulation ; LacZ fusion protein ; Northern hybridization ; Regulatory circuit ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mode of expression was investigated for two positive regulatory genes PHO2 and PHO4, whose products are indispensable for the transcriptional control of the structural genes of repressible acid phosphatase and the inorganic phosphate (Pi) transport system in Saccharomyces cerevisiae. Northern analysis of poly(A)+ RNA of the wild-type and the pho regulatory mutants with PHO4 DNA as hybridization probe and expressional analysis of a pho4′-'lacZ fused gene on a YEp plasmid revealed that PHO4 is expressed at a low level, constitutively, and independently of the PHO regulatory system and Pi in the medium. Similar analyses with PHO2 DNA indicated that PHO2 is expressed at an even lower level than PHO4, and is repressed by Pi and by the active PHO2 product, possibly at the translational level, while retaining a substantial level of basal activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330939
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  • 2
    Electronic Resource
    Electronic Resource
    A cis-acting locus for the stable propagation of yeast plasmid pSR1 (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 355-360 
    Details
    ISSN: 1617-4623
    Keywords: pSR1 plasmid ; Stability control ; cis-acting locus ; Z locus ; Zygosaccharomyces rouxii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A DNA plasmid resembling 2 μm DNA of Saccharomyces cerevisiae, pSR1, isolated from a strain of Zygosaccharomyces rouxii, has a cis-acting region, Z, for plasmid stability. The Z region was delimited to a sequence of at most 383 bp in a small unique region of the plasmid. The Z region is high in A:T pairs and contains three different pairs of short (ca. 25 bp) inverted repeats with 65% to 79% homology and three copies of direct repeats of 24 to 27 bp in length with 67% to 72% homology, but does not encode a noteworthy open reading frame. It was suggested that the Z region interacts with the S product(s) encoded by the same plasmid and with a specific host factor, but not with the other stabilization factor encoded by the P locus on the sPR1 molecule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331601
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  • 3
    Electronic Resource
    Electronic Resource
    Factors encoded by and affecting the holding stability of yeast plasmid pSR1 (1987)
    Springer
    Molecular genetics and genomics 206 (1987), S. 88-94 
    Details
    ISSN: 1617-4623
    Keywords: pSR1 plasmid ; Stability control ; Functional region ; Zygosaccharomyces rouxii ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 2 μm DNA-like plasmid, pSR1, isolated from a strain of Zygosaccharomyces rouxii has three coding frames, P, S and R. Insertional inactivation of R completely abolished the intramolecular recombination, and the defect was complemented by an intact R frame on a coexistent plasmid molecule. The P and S regions were also transactive and important, but not essential, for the stable maintenance of the plasmid molecules. Insertional disruption of the P frame suggested that it produces a protein factor. Similar insertional disruption of the S frame affected the plasmid stability in Z. rouxii and Saccharomyces cerevisiae hosts differently, depending on whether the inserted DNA fragment was a short 8 bp SalI linker or a long (2.2 kb) DNA fragment. Results strongly suggested that the S region encodes two factors, one RNA and the other a protein, and that the S protein is compatible with a sprecific hostfactor in Z. rouxii, but not in S. cerevisiae. In addition, a cis-acting locus, Z, was found at a site in the plasmid molecule where no distinct open reading frames were located. No long direct repeats or inverted repeats were observed in the Z region, such as are found in the REP3 locus of 2 μm DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326541
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  • 4
    Electronic Resource
    Electronic Resource
    A gene, SMP2, involved in plasmid maintenance and respiration in Saccharomyces cerevisiae encodes a highly charged protein (1993)
    Springer
    Molecular genetics and genomics 236 (1993), S. 283-288 
    Details
    ISSN: 1617-4623
    Keywords: Plasmid stability ; pSR1 plasmid ; rho mutation ; smp mutation ; Yeast plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The smp2 mutant of Saccharomyces cerevisiae shows increased stability of the heterologous plasmid pSR1 and YRp plasmids. A DNA fragment bearing the SMP2 gene was cloned by its ability to complement the slow growth of the smp2 smp3 double mutant (smp3 is another mutation conferring increased stability of plasmid pSR1). The nucleotide sequence of SMP2 indicated that it encodes a highly charged 95 kDa protein. Disruption of the genomic SMP2 gene resulted in a respiration-deficient phenotype, although the cells retained mitochondrial DNA, and showed increased stability of pSR1 like the original smp2 mutant. The fact that the smp2 mutant is not always respiration deficient and shows increased pSR1 stability even in a rho 0 strain lacking mitochondrial DNA suggested that the function of the Smp2 protein in plasmid maintenance is independent of respiration. The SMP2 locus was mapped at a site 71 cM from lys7 and 21 cM from ilv2/SMR1 on the right arm of chromosome XIII.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277124
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  • 5
    Electronic Resource
    Electronic Resource
    A specific host factor binds at a cis-acting transcriptionally silent locus required for stability control of yeast plasmid pSR1 (1993)
    Springer
    Molecular genetics and genomics 238 (1993), S. 120-128 
    Details
    ISSN: 1617-4623
    Keywords: Cis-acting locus ; Plasmid stability ; pSR1 plasmid ; Yeast plasmid ; Z locus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cis-acting locus, Z, of plasmid pSRl functions in stable maintenance of the plasmid in the native host, Zygosaccharomyces rouxii. The Z locus was shown to be located in a 482 by sequence in the 5′ upstream region of an open reading frame, P, by subcloning various DNA fragments in a plasmid replicating via the ARS1 sequence of the Saccharomyces cerevisiae chromosome. Northern analysis revealed that the Z region is not transcribed in either the native host Z. rouxii or the heterologous host S. cerevisiae. The Z region is protected from microccocal nuclease attack in Z. rouxii but not in S. cerevisiae, its protection depending on the product of the S gene encoded by pSR1. Gel retardation assays suggested that a factor present in nuclear extracts of Z. rouxii cells, irrespective of the presence or absence of a resident pSRI plasmid, binds to a 111 by Rsal-Sacll sequence in the Z region. These findings suggest that a host protein binds to the Z locus and that the S product interacts with this DNA-protein complex and stabilizes pSRl.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279538
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  • 6
    Electronic Resource
    Electronic Resource
    Function of the PHO regulatory genes for repressible acid phosphatase synthesis in Saccharomyces cerevisiae (1989)
    Springer
    Molecular genetics and genomics 217 (1989), S. 40-46 
    Details
    ISSN: 1617-4623
    Keywords: Gene dosage ; Gene expression ; Regulatory circuit ; Signal transmission ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Expression of the repressible acid phosphatase (rAPase) gene, PHO5, of Saccharomyces cerevisiae is repressed by a certain level of inorganic phosphate (Pi) in the medium and is derepressed when the Pi concentration is lowered. The Pi signals are conveyed to PHO5 by a regulatory system consisting of proteins coded for by the PHO2, PHO4, PHO80 and PHO81 genes. We have found that the transcription of PHO81 is regulated by Pi through the PHO regulatory system. Increasing the dosage of PHO4 and PHO81 by ligating each gene to YEP13 gives rise to, respectively, considerable and weak synthesis of rAPase by cultivation of the transformants in high-Pi medium; but in low-Pi medium, increased dosage of PHO4 stimulates the rAPase synthesis significantly, whereas PHO81 has no effect. Increased dosage of PHO2 stimulates rAPase synthesis considerably in low-Pi but not in high-Pi. A coordinate increase of PHO80 cancels the dosage effect of PHO4, but not that of PHO81. Coordinate increases of PHO80 and PHO2 give rise to the same phenotype as an increased dosage of PHO80 alone. The level of the PHO4 protein was found to be the limiting factor of the rAPase synthesis and the copy number of the PHO5 gene not to be. These facts accord with the idea that the PHO80 protein transmits the Pi signals to the PHO5 gene via the PHO4 protein, whereas the PHO2 protein does not have a direct function in the signal transmission.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330940
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