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  • 1
    Electronic Resource
    Electronic Resource
    The bialaphos biosynthetic genes ofStreptomyces hygroscopicus: Molecular cloning and characterization of the gene cluster (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 42-53 
    Details
    ISSN: 1617-4623
    Keywords: Streptomyces ; Antibiotics ; Bialaphos ; Resistance gene ; Biosynthetic gene cluster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated and studied the organization ofStreptomyces hygroscopicus genes responsible for the biosynthesis of the antibiotic herbicide bialaphos. Bialaphos production genes were cloned from genomic DNA using a plasmid vector (pIJ702). Three plasmids were isolated which restored productivity toS. hygroscopicus mutants blocked at different steps of the biosynthetic pathway. Subcloning experiments using other nonproducing mutants showed that four additional bialaphos production genes were also contained on these plasmids. A gene conferring resistance to bialaphos, which was independently cloned using the plasmid vector pIJ61, and an antibiotic-sensitive host (S. lividans), was also linked to the production genes. Cosmids were isolated which defined the location of these genes in a 16 kb cluster.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02428031
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transcriptional analysis of groEL genes in Streptomyces coelicolor A3(2) (1994)
    Springer
    Molecular genetics and genomics 245 (1994), S. 61-68 
    Details
    ISSN: 1617-4623
    Keywords: groE ; Streptomyces ; Gene expression RNA polymerase ; DNA-binding protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Streptomyces coelicolor A3(2), synthesis of the groES, groES-groEL1 and groEL2 transcripts is induced either by heat shock or by undefined physiological stress signals present at a certain stage of growth. Under all conditions tested, transcription of groES and groES-groEL1 orginated from a unique start site upstream of groES, whereas transcription of groEL2 originated from a unique site upstream of groEL2. RNA polymerase isolated either from heat-shocked or control mycelia allowed in vitro transcription from the P1 promoter of groES/EL1 and the P2 promoter of groEL2. The fact that these two RNA polymerase preparations both initiated transcription with equal efficiency from the same sites suggested that a heat shock-specific σ factor is not responsible for the temperature-induced transcription of groE genes. Instead, regulation of these genes from vegetative-type promoters may be effected by a DNA-binding protein observed in gel retardation assays, which recognizes a motif found in the groE and dnaK promoter regions of many prokaryotic genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279751
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of procaryotic developmental stages by statistical analyses of two-dimensional gel patterns (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 1418-1428 
    Details
    ISSN: 0173-0835
    Keywords: Two-dimentional polyacrylamide gel electrophoresis ; Streptomyces ; Development ; Multivariate ; Regulation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Multivariate statistical comparisons of two-dimensional protein (2-D) gel patterns were used for the first time to define stages of a biological developmental system. The differentiating procaryote, Streptomyces coelicolor, was radiolabeled in liquid cultures at 16 intervals during development, and radioactive proteins were separated and quantified on 2-D gels. Cluster, principal component, and correlation analyses classified these gel patterns into four distinct groups, each reflecting a pattern of gene expression specific for a stage of development. These studies focused our attention on a phase of arrested growth as a key regulatory transition leading to secondary metabolism and a phase of renewed growth. Proteins whose synthesis was switched on or off during the “transitional” phase (some 21 and 18, respectively) were identified and will be the focus of future studies designed to identify their physiological or regulatory function.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150180817
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