GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    The nucleotide sequence of a transposable haloalkanoic acid dehalogenase regulatory gene (dehR I) fromPseudomonas putida strain PP3 and its relationship with σ54-dependent activators (1995)
    Springer
    Biodegradation 6 (1995), S. 247-255 
    Details
    ISSN: 1572-9729
    Keywords: Pseudomonas putida ; dehalogenase I regulator sequence ; σ54-dependent activator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The mobile genetic element,DEH found inPseudomonas putida PP3 carries a 2-haloalkanoic acid dehalogenase structural gene,dehI, and its associated regulatory gene,dehR I. The nucleotide sequence ofdehR I was determined. The gene had an open reading frame putatively encoding for a 64 kDa protein containing 571 amino acid residues. The protein was similar to previously published sequences of several other σ54-dependent activator proteins. Amino acid sequence analysis showed that the deduced DehRI protein clustered with the NifA nitrogenase regulatory activator family, and was most closely related, with 47.7% similarity, to a ‘NifA-like’ deduced partial sequence from a plasmid-encoded ORF inPseudomonas sp. strain NS671, associated with L-amino acid production. The domain structure of DehRI was analysed by alignment with other NifA-like and NtrC-like sequences and showed a highly conserved central region of approximately 230 amino acids, and a potential DNA-binding domain. No homology was detected between the deduced DehRI and other σ54-dependent activator sequences at the N-terminus, a result which was consistent with that region being the domain which recognised inducer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00700464
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Cryptic dehalogenase and chloroamidase genes in Pseudomonas putida and the influence of environmental conditions on their expression (1995)
    Springer
    Archives of microbiology 163 (1995), S. 57-64 
    Details
    ISSN: 1432-072X
    Keywords: Pseudomonas putida ; Dehalogenase ; Chloroamidase ; Cryptic genes ; Mutations in microbial colonies ; Decryptification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mutants of two strains of Pseudomonas putida expressed two cryptic chloroamidases (C-amidase and Hamidase) and one cryptic dehalogenase (DehII). The mutants were selected on either 2-chloropropionamide (2CPA) or 2-monochloropropionate (2MCPA), developing as papillae in parental colonies growing on a metabolisable support substrate. Mutants expressing C-amidase were selected if 2CPA was utilised as either a carbon or a nitrogen source. H-amidase mutants were selected only if 2CPA was used as a nitrogen source. Growth temperature and pH affected the frequency of papillae production, although different temperatures and pHs did not affect the overall growth characteristics of the parental colonies. Decreasing growth temperature increased the frequency of 2cpa+ papillae formation, but decreased the frequency of 2mcpa+ papillae formation. Low pH (6.0) prevented the formation of 2mcpa+ and 2cpa+ papillae. However, in the case of the 2cpa+ papillae, decreasing the growth temperature also allowed papillae formation at pH 6.0.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262204
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Environmentally directed mutations in the dehalogenase system of Pseudomonas putida strain PP3 (1992)
    Springer
    Archives of microbiology 158 (1992), S. 176-182 
    Details
    ISSN: 1432-072X
    Keywords: Pseudomonas putida ; Dehalogenase ; Halogenated alkanoic acid ; Resistance to halogenated alkanoic acid ; Environmentally directed mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Favourable mutations involving the two dehalogenases (DehI and DehII) of Pseudomonas putida PP3 and derivative strains containing the cloned gene for DehI (dehI) occurred in response to specific environmental conditions, namely: starvation conditions; the presence of dehalogenase substrates (halogenated alkanoic acids — HAAs) which were toxic to P. putida; and/or the presence of a potential growth substrate. Fluctuation tests showed that these mutations were environmentally directed by the presence of HAAs. the mutations were associated with complex DNA rearrangements involving the movement of dehI located on a transposon DEH. Some mutations resulted in switching off the expression of either one or both of the dehalogenases, events which were effective in protecting P. putida from toxic compounds in its growth environment. Other mutations partially restored P. putida's dehalogenating capability under conditions where toxic substrates were absent. Restoration of the capability to untilize HAAs was favoured when normal growth substrates were present in the environment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290813
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Interactions between populations ofPseudomonas putida leading to the expression of a cryptic dehalogenase gene (dehll) (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 186-192 
    Details
    ISSN: 1573-0972
    Keywords: Biodegradation ; cryptic genes ; dehalogenases ; papillae ; Pseudomonas putida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In appropriate environments containing 2-monochloropropionic acid (2MCPA), mutations in a population of nondehalogenatingPseudomonas putida, strain PP40-040 (parent population), resulted in the formation of 2mcpa+ papillae as a result of the decryptification of adehII gene. Increasing the size of the parent population, for example by increasing the availability of a metabolizable substrate such as succinate or lactate, increased the number of 2mcpa+ papillae formed because there were more parent cells available for mutation to the 2mcpa+ phenotype. The presence of a dehalogenating population, such asP. putida strain PP3, in close proximity to the non-dehalogenating population, also increased the number of 2mcpa+ papillae formed. This was due to the excretion of dehalogenases into the growth medium, which caused localized dehalogenation of the available 2MCPA, yielding a metabolizable substrate. This substrate stimulated the growth of the non-dehalogenating population, in turn increasing the number of 2mcpa+ papillae formed. Barriers, such as dialysis membranes, which prevented the excretion of the dehalogenases into the growth medium, prevented the stimulation of 2mcpa+ papillae formation by preventing release of metabolizable substrates from 2MCPA breakdown. Cell-free extracts (CFE) from dehalogenase-producing populations had a similar effect for the same reason. CFE without dehalogenase activity or in which the dehalogenase activity had been destroyed by heating failed to stimulate parent population growth and 2mcpa+ papillae formation. In the case ofPseudomonas putida strain PP3, which carries an easily transposed dehalogenase-encoding transposon, treatment of CFE with DNAase eliminated an additional factor involved in the formation of 2mcpa+ papillae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00704646
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...