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  • 1
    Electronic Resource
    Electronic Resource
    P-31 in-vivo NMR investigation on the function of polyphosphates as phosphate-and energysource during the regreening of the green alga Chlorella fusca (1989)
    Springer
    Archives of microbiology 152 (1989), S. 167-171 
    Details
    ISSN: 1432-072X
    Keywords: Chlorella fusca ; Green alga ; Nitrogen starvation ; Polyphosphates ; P-31 in-vivo NMR spectroscopy ; Regreening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The green alga Chlorella fusca accumulates polyphosphates under conditions of nitrogen starvation while deassembling the photosynthetic apparatus. The polyphosphate content of cells regreening after resupply with nitrate under different culture conditions was investigated by P-31 in-vivo NMR spectroscopy. Neither phosphate deficiency nor anaerobiosis during the first hours of regreening inhibited the recovery of the cells. Polyphosphates were degraded during regeening. Differences in the amount of polyphosphates of phosphate supplied and deficient cells occurred only after more then 8 h. After 16 h phosphate deficient cells had still 75% of the polyphosphate content of phosphate suppled cells. In cells kept under anaerobic conditions polyphosphate degradation was much higher than in oxygen supplied cells. After 8 h they contained less than 50% of the polyphosphate content of oxygen supplied cells. These data suggest that polyphosphates serve as obligatory phosphate source during regreening and may be used as an energy source.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00456096
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Distribution of polyphosphates in cell-compartments of Chlorella fusca as measured by 31P-NMR-spectroscopy (1986)
    Springer
    Archives of microbiology 144 (1986), S. 48-54 
    Details
    ISSN: 1432-072X
    Keywords: Chlorella fusca ; Green alga ; 31P-NMR-spectroscopy ; Polyphosphates ; Cellular localization ; Metal-ion complexation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In suspensions of the green alga Chlorella fusca the influence of high pH and high ethylene-diamine-tetraacetic acid concentrations in the external medium, of French-press and perchloric acid extraction of the cells and of alkalization of the intracellular pH on the polyphosphate signal in 31P-nuclear magnetic resonance (31P NMR) spectra was investigated. The results show that part of the polyphosphates of asynchronous Chlorella cells are located outside the cytoplasmic membrane and complexed with divalent metal-ions. These polyphosphates are tightly bound to the cell wall and/or the cytoplasmic membrane and are not susceptible to hydrolyzation by strong acid at room temperature, in contrast to the intracytoplasmic polyphosphates. Upon alkalization of the internal pH of Chlorella cells, polyphosphates, previously not visible in the spectra become detectable by 31P-NMR-spectroscopy. 31P-NMR spectroscopic monitoring of polyphosphates during gradual alkalization of the extra-and intracellular space is proposed as a quick method for the estimation of the cellular polyphosphate content and distribution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00454955
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    Paper (German National Licenses)
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