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  • 1
    ISSN: 1432-2285
    Keywords: Picea abies ; Cytogenetic bioindication ; Chromosomal aberrations ; Soil ; Ozone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The classification of chromosomal aberrations was used to characterize different factors affecting chromosomes in the root meristem of Norway spruce [Picea abies (L.) Karst.] trees. It is important to know the most significant factor affecting the chromosomes in the root meristem of plants at natural sites. The results suggest that an intensive site effect is more significant than the soil or the provenance of the individual. This cytogenetic plant test system was also used to investigate 5-year-old spruce trees exposed in environmental chambers to elevated concentrations of carbon dioxide (750 cm3m−3) and ozone (0.08 cm3m−3) as single variables or in combination, and then transferred to a field for observation of a “memory effect”. The fumigated variants showed an increased number of chromosomal aberrations compared to the controls, which carried on as a memory effect in the root meristems far beyond the fumigation period.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 76 (1988), S. 294-297 
    ISSN: 1432-1939
    Keywords: Thiols ; Glutathione reductase ; Osmotic value ; Annual rhythm ; Picea abies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We determined the water-soluble thiol (SH) content and the osmolality of the cell sap in spruce trees of both low- and high-altitude origin that were transplanted to two test areas at different altitudes (400 m and 1700 m). There was little or no significant difference in SH content and osmotic value between trees of low- or high-altitude origin at the same altitude, but the sulfhydryl (SH) content varied with the altitude of cultivation, being higher at 1700 m than at 400 m. SH content and osmotic value were higher in winter, independent of altitude and provenance. The annual pattern of water-soluble and protein-bound SH content and glutathione reductase activity was measured in spruce trees at 1700 m; these values also peaked in winter and were lowest in summer.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1615-6102
    Keywords: Glutathione ; Root ; Chromosomal aberration ; Ultrastructure ; Picea abies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Young spruce seedlings (Picea abies [L.] Karst.) grown in hydroponic culture were exposed to three different concentrations (50,100, and 500 μM) of reduced glutathione for 24 h. These physiologically relevant concentrations of glutathione had a multiple effect on the investigated tissue. Feeding of glutathione to roots increased the concentrations of thiols (glutathione, cysteine, and γ-glutamyl-cysteine) in roots, decreased the rate of cell divisions, induced mitotic abnormalities, and affected the cell ultrastructure. Electron micrographs showed effects such as advanced vacuolation, dilated rough-endoplasmic-reticulum cisternae, and separations of the plasma membrane from the cell wall.
    Type of Medium: Electronic Resource
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