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  • 1
    Electronic Resource
    Electronic Resource
    The structure of the eye of Sardinops caerulea, Engraulis mordax, and four other pelagic marine teleosts (1963)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 113 (1963), S. 287-329 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051130214
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  • 2
    Electronic Resource
    Electronic Resource
    Angiogenic potential of the avian somite (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Developmental Dynamics 202 (1995), S. 165-171 
    Details
    ISSN: 1058-8388
    Keywords: Somite ; Quail ; Chick ; Chimera ; Angiogenesis ; Bilaterality ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: We have studied the angiogenic potential of the unsegmented paraxial mesoderm and epithelial somites of the trunk with homotopical grafts between quail and chick embryos. Quail endothelial cells of the grafts were stained with the QH-1 antibody after 1-6 days of reincubation. The unsegmented paraxial mesoderm and all parts of the epithelial somite were found to contain angioblasts which develop into QH-1 positive endothelial cells. These cells are incorporated into the lining of the host's blood vessels such as the perineural vascular plexus and the dorsal branches of the aorta. There is a certain preference as concerns the location of endothelial cells derived from different parts of the somites. Angioblasts from ventral somite halves are mainly found in ventrolateral blood vessels. Those from dorsomedial quadrants form vessels in the dermis of the back, and those from dorsolateral quadrants can be found in the ventrolateral body wall and the wing. With the exception of the dorsal perineural vascular plexus, angioblasts do not cross the median plane of the body. This shows that, although angioblasts migrate extensively, there is bilaterality of the vascular system in the trunk. It remains to be studied whether the notochord plays a role in the establishment of this bilaterality. © 1995 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1002020208
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  • 3
    Electronic Resource
    Electronic Resource
    Sensitive stages for the production of developmental abnormalities in mice with 5-fluorouracilThis investigation was supported, in part, by PHS research grants RG-4827 and RG-5300 from the National Institutes of Health, Public Health Service, and in part by Contract AT (30-1) -1762 with the U. S. Atomic Energy Commission. (1960)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 106 (1960), S. 89-96 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001060202
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  • 4
    Electronic Resource
    Electronic Resource
    Changes in dorsoventral but not rostrocaudal regionalization of the chick neural tube in the absence of cranial notochord, as revealed by expression of Engrailed-2 (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 193 (1992), S. 389-396 
    Details
    ISSN: 0002-9106
    Keywords: Positional information ; Neurulation ; Pattern formation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Notochord has been implicated in previous studies in both the dorsoventral and rostrocaudal patterning of the developing neural tube. This possibility has been further explored by analyzing the expression of Engrailed-2 in chick embryos developing with cranial notochord defects. Control embryos containing intact notochords expressed Engrailed-2 protein within the neural tube and in a subset of the neural crest and overlying surface ectoderm at the future mesencephalon and cranial metencephalon levels. Within the neural tube, expression was confined to cell nuclei in the roof plate and lateral walls; floor plate nuclei directly overlying the notochord typically failed to show expression. After surgical removal of Hensen's node, the source of notochord precursor cells, embryos were cultured through neurulation and assayed for expression of Engrailed-2 protein. All embryos that partially or completely lacked cranial notochord expressed Engrailed-2 in a pattern similar to that of control embryos containing intact notochords, except that when notochord and floor plate were absent, Engrailed-2 was also expressed in the most ventral part of the neural tube. These results indicate that (1) Engrailed-2 expression is suppressed in the most ventral neural tube owing to induction of the floor plate by the notochord, and (2) that the presence of an underlying notochord is not required for correct rostrocaudal expression, suggesting that multiple pathways act in the patterning of the rudiment of the central nervous system. © 1992 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001930411
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  • 5
    Electronic Resource
    Electronic Resource
    Changes in blood sugar and tissue glycogen in the hamster during arousal from hibernationThis research was supported in part by Air Force Contract No. A F 33 (038)-18133 and in part by the Milton Fund of Harvard University. (1953)
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 41 (1953), S. 471-491 
    Details
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1030410307
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  • 6
    Electronic Resource
    Electronic Resource
    The effect of temperature on the isolated hearts of closely related hibernators and non-hibernatorsThis research was supported in part by the U. S. Air Force under Contract AF41 (657)-190 monitored by the Arctic Aeromedical Laboratory, APO 731, Seattle, Washington, and in part by U.S.P.H.S. grant RC-5197. (1959)
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 54 (1959), S. 53-63 
    Details
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1030540107
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  • 7
    Electronic Resource
    Electronic Resource
    Structure of transcriptionally active chromatin: Radiological evidence for requirement of torsionally constrained DNA (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 141 (1989), S. 346-352 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Synthesis of α- and β-globin RNA in DMSO-induced Friend's erythroleukemia cells and synthesis of immunoglobulin γ- and k-chain RNA, total RNA, 5S RNA, and tRNA in mouse myeloma cells (MPC-11) was inhibited by γ-irradiation. For all RNA species, synthesis decreased nearly exponentially as a function of radiation dose, whereas RNA size distributions, turnover rates, and specific activities of radioactively labeled RNA were affected only insignificantly. D37 values for the loss of synthesis of various RNA species correspond to target sizes ranging from 21,000 to 53,000 kd, or 30-80 kbp of DNA. These target sizes are several-fold larger than the structural genes in question; however, they correspond well with the size of DNA loops, or “domains” constrained by the nuclear matrix. The data suggest that the eukaryotic transcription unit is the torsionally constrained chromatin loop, transcription of which may be inactivated, or significantly reduced by a DNA single-strand break.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041410216
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  • 8
    Electronic Resource
    Electronic Resource
    Efficient secretion in yeast based on fragments from K1 killer preprotoxin (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 8 (1992), S. 261-272 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces crevisiae ; killer yeast ; protein secretion ; heterologous gene expression ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The α and β components of the secreted K1 killer toxin of Saccharaomyces cerevisiae are derived from residues 45-147 and 234-316, respectively, of the 316 residue prepotoxin (ppTox). The β N-terminus is produced by Kex2 cleavage after Lys Arg233, when β1a(the mature sequence of β-lactamase)is fused at this site and the fusion is expressed form the PGK promoter in pDT17, a multicopy plasmid, unexpectedly modest levels of βla secretion resulted. Over-expression of Kex2 failed to increase βla secretion while a kex2-null mutation reduced secretion by 98%. βla secretion in a Kex+ strain was not enhanced by inactivation of the a toxin component or by deletion of most of its central hydrophobic segments. However SP-βla, produced by deletion of ppTox residues 35-176, expressed 10-fold higher βla activity and the precursor was not secreted with similar efficiency in a kex - 2 null strain. Fusions of βla to ppTox at Ala34 or Ala46 also led to efficient secretion in both KEX2 and kex - 2-null strains. Since these βla fusions differ only in segments well downstream of the signal peptide and all had similar transcript levels, the efficiency of βla secretion is apparently determined by the efficiency with efficiency with which these fusions are translocated to the Golgi compartment where Kex2 is active. Efficiency is high for the shorter fusions but is 10% or less for the longer fusions; even this fraction is apparently diverted to the vacuole if not cleaved by Kex2. SP-βla was athe most efficient construct tested; secreted βla reacahed 4% of total cell protein, modestly exceeding levels produced by fusion to the MFα1-encoded preproα-factor, suggesting potential for the production of foreign proteins in yeast.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320080404
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  • 9
    Electronic Resource
    Electronic Resource
    Use of β-lactamase as a secreted reporter of promoter function in yeast (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 497-508 
    Details
    ISSN: 0749-503X
    Keywords: Protein secretion and processing ; gene expression ; killer toxin ; Kex2 protease ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: K1 preprotoxin is the 316 residue precursor of the K1 killer toxin secreted by the yeast Saccharomyces cerevisiae. The SPβla reporter consists of the mature, secreted form of β-lactamase (βla) fused to S and P, two fragments of preprotoxin. S is the N-terminal 34 residues, including the secretion signal. P, a 67 residue ‘processing’ segment with three sites for N-glycosylation, terminates in a Lys Arg site for cleavage by the Kex2 protease. Expression of SPβla in yeast results in efficient secretion, processing by signal peptidase and glycosylation in the endoplasmic reticulum, producing proßla. Kex2 cleavage of proßla in the lumen of a late Golgi compartment releases βla, which accumulates stably in culture media buffered at pH 5·8-7. The half-life of secretion is 11 min at 30°C; 10-12% of the total activity in exponential-phase cells is intracellular, mostly in the form of proßla, indicating that transit from the endoplasmic reticulum to the Golgi is rate limiting. We have used SPβla expression in single- and multi-copy vectors to compare the PGK, GAL1, GAL10, PHO5 and CUP1 promoters under varying nutritional conditions. In exponential-phase cells, secretion of βla over a 40-fold range and up to several μg/ml was proportional to transcript level, demonstrating that SPβla can be employed as a convenient secreted reporter of promoter function in yeast.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320100409
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  • 10
    Electronic Resource
    Electronic Resource
    The relative importance of transcriptional and post transcriptional regulation of Drosophila chorion gene expression during oogenesis (1991)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 12 (1991), S. 196-205 
    Details
    ISSN: 0192-253X
    Keywords: Run-on transcription ; developmentally regulated gene expression ; follicle cell nuclei ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: To determine the relative roles of transcriptional and post-transcriptional events in establishing the temporal pattern of chorion gene expression in Drosophila, we have examined chorion gene transcription, RNA accumulation, and protein synthesis in follicles of selected pre-early- and late-choriogenic stages. Chorion gene transcription was assayed in follicle cell nuclei by nuclear run-on reactions. For the s 15, s 16, s 18, s36, and s38 chorion genes, the periods of intense transcription are as predicted from the dynamics of RNA accumulation and protein synthesis, indicating that these genes are primarily regulated at the transcriptional level. In contrast, gene s19 appears subject to post-transcriptional control at stage 14, when transcription rates are substantially higher than predicted from the observed RNA levels.Transcription of regions between the clustered and tandemly oriented chorion genes was also examined. In contrast to many RNA polymerase II transcribed genes, for the s18 and s36 chorion genes run-on transcription appears to terminate within about 100 base pairs downstream of the polyadenylation sites, corroborating previous reports based on electron microscopy of s36 [Osheim et al., EMBO J 5:3591-3596, 1986].
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020120304
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