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  • Life and Medical Sciences  (1)
  • acyl-CoA ligase (AMP forming)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Brassica napus cDNAs encoding fatty acyl-CoA synthetase (1997)
    Springer
    Plant molecular biology 33 (1997), S. 911-922 
    Details
    ISSN: 1573-5028
    Keywords: acyl-CoA ligase (AMP forming) ; AMP-binding protein ; enzymatic activity ; fatty acid ; northern blot ; sequence comparison
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract From a cDNA library of developing siliques of rapeseed (Brassica napus L.) we have isolated five full-length clones encoding polypeptides of the AMP-binding protein family. Two cDNAs encode fatty acyl-CoA synthetase activity (EC 6.2.1.3). The deduced polypeptides share about 52% identical amino acids. After expression in Escherichia coli the predicted enzymatic activity was confirmed by in vitro assays and product analysis. The enzymatic activity for one of the clones was characterized in detail by determination of the K m for oleic acid (10.4 µm) and the pH optimum (between 7 and 8). For the three additional clones no enzymatic activities could be demonstrated after expression in E. coli, although two of them exhibit similarity to either eukaryotic or prokaryotic acyl-CoA synthetases. The sequences are compared to a number of related expressed sequence tags from Brassica and Arabidopsis. Potential subcellular locations and functions of the deduced polypeptides within plant cells are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005780529307
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  • 2
    Electronic Resource
    Electronic Resource
    Membranlipid-Biosynthese in Chloroplasten (1983)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 13 (1983), S. 178-187 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.19830130607
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    Paper (German National Licenses)
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