ISSN:
1573-9368
Keywords:
human β globin
;
3′ enhancer
;
transgenic
;
promoter
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Our interest in thecis-acting elements that promote the up-regulation of the β globin gene has led to a systematic deletion analysis of portions of the β globin gene in the context of the HS2 and γ globin gene using transgenic mice. In constructs that delete the 5′ region to only 265 bp, high-level erythroid-specific expression was observed. Further deletion to 122 bp, however, results in significantly reduced expression levels A substitution of a minilocus control region for the single HS2 site was also produced, resulting in increased β globin expression over that seen with the HS2 alone. These results are consistent with the presence of an enhancer-like element between −122 and −265. In addition, a construct in which the entire β globin gene promoter was replaced by a thymidine kinase promoter was tested. Interestingly, no expression was detected in these transgenic mice. This may indicate the requirement for an erythroid-specific promoter to drive this gene. Finally, the 3′ region of the β globin gene was deleted in order to examine the effect of a previously defined 3′ enhancer region. With deletion of this region, the expression of the human β globin gene in transgenic mice is unchanged relative to the parental constructs.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01972878
Permalink
