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  • 1
    Online Resource
    Online Resource
    Cham : Springer International Publishing | Cham : Imprint: Springer
    Keywords: Microbial ecology. ; Ecology . ; Soil science. ; Microbial genetics. ; Environment. ; Earth sciences.
    Description / Table of Contents: Chapter 1: A Brief Introduction to Hot Desert Environments: Climate, Geomorphology, Habitats and Soils -- Chapter 2: Novel methods for studying the structure and function of hot desert microorganisms and their communities -- Chapter 3: Phototrophic Mats of the Desert: The Bacteria of the Biological Soil Crust Community- Chapter 4: Microbial Ecology of Hot Desert Soils -- Chapter 5: Biology of Desert Endolithic Habitats -- Chapter 6: Journey of a thousand miles: The evolution of our understanding of viruses in hot Deserts -- Chapter 7: C, N and P nutrient cycling in Drylands -- Chapter 8: Diversity and plant growth promoting properties of microbiomes associated with plants in desert soils -- Chapter 9: Insights of Extreme Desert Ecology to the Habitats and Habitability of Mars -- Chapter 10: Survival under stress: Microbial adaptation in hot desert soils -- Chapter 11: The response of soil microbial communities to hydration and desiccation cycles in hot desert ecosystems -- Chapter 12: Hot Desert Microbiology: Perspectives in a Warming World.
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource(XVI, 349 p. 49 illus. in color.)
    Edition: 1st ed. 2022.
    ISBN: 9783030984151
    Series Statement: Ecological Studies, Analysis and Synthesis 244
    Language: English
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  • 2
    ISSN: 1432-0983
    Keywords: Key words Yeast ; Heterologous gene expression ; Sulfolobus ; Hyperthermophile phosphoglycerate kinase ; Archaea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The gene encoding phosphoglycerate kinase (PGK) from the Archaeon Sulfolobus solfataricus, an organism growing optimally at 87 °C, was inserted into a yeast expression vector under the control of the galactose-inducible GAL1 yeast promoter. This vector was then transformed into a pgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants were obtained on galactose plates at 37 °C, but not 28 °C. These transformants contained low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast, a mesophile, therefore enabled complementation of the yeast pgk defect at 37 °C but not at 28 °C.
    Type of Medium: Electronic Resource
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