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  • 1
    Electronic Resource
    Electronic Resource
    Effect of isoelectric point on biodistribution and inflammation imaging with indium-111-labelled IgG (1990)
    Springer
    European journal of nuclear medicine 17 (1990), S. 305-309 
    Details
    ISSN: 1619-7089
    Keywords: Indium ; IgG ; Diethylene triamine penta-acetic acid ; Infection ; Isoelectric point ; Radiolabelledantibody ; Biodistribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Electrostatic effects play an important role in protein interactions and may alter the biodistribution of antibodies. To study the effect of molecular charge on the biodistribution and infection imaging properties of human polyclonal immunoglobulin G (IgG), its iso electric point was varied by changing the level of diethylene triamine penta-acetic acid (DTPA) substitution: 0.8, 0.9, 3.7, 5.1 and 5.9 DTPA/IgG. Biodistributions of the different IgG preparations were determined at 10 min, 1, 6, 24, and 48 h post injection in normal rats, and infection imaging properties were determined in rats withEscherichia coli thigh infections. The biodistribution was significantly affected by pl. The immunoglobulin preparations with 0.9 and 3.7 DTPA/IgG showed faster clearance from the circulation and generally lower accumulation in most organs. The images had a target-to-background ratio of approximately 1.3–2.3:1. These results suggest that even though targeting is not affected by the level of DTPA substitutions, preparations with 0.9 and 3.7 DTPA/IgG may be superior imaging agents because of reduced accumulation by background organs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01268020
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Accumulation of immunoglobulin G at focal sites of inflammation (1992)
    Springer
    European journal of nuclear medicine 19 (1992), S. 159-165 
    Details
    ISSN: 1619-7089
    Keywords: Infection ; Immunoglobulin ; IgG ; Rate constants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To evaluate the factors responsible for the accumulation indium-111 immunoglobulin Gr (111InIgG) at sites of inflammation, sequential measurements of tissue blood volume, interstitial fluid volume and accumulation of radiolabelled albumin and IgG were made in rats following Escherichia coli infection in the thigh. Compared with normal thigh muscle, there was ∼two-fold increase in interstitial fluid volume and ∼ 1.5-fold increase in plasma and red blood cell volumes in infected muscle. For both proteins, there was a fivefold increase in influx rate constant (kin) in infected muscle. In normal muscle, the interstitial fluid concentration of labelled human serum albumin (111In-HSA) was significantly higher than that of 111In-IgG (P〈0.01). In contrast, the concentrations in infected muscle were nearly identical. The concentration ratios (infected to normal muscle) were 1.7:1 for HSA and 3:1 for IgG. These data suggest that the infection imaging properties of 111In-IgG are related to expansion of the space available to macromolecules in infected tissue and increased transport into this space. At clinically important imaging times (24–48 h after injection), the higher target-to-background ratio of 111In-IgG compared with 111In-HSA is not due to the higher accumulation IgG in infected tissue but rather to the higher accumulation of HSA in normal tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00173275
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    Paper (German National Licenses)
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