ISSN:
1432-069X
Keywords:
Hydroxyethylstarch
;
Phagocytosis
;
Keratinocytes
;
Monocytes
;
Flow cytometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Severe itching for unknown reasons has been reported after administration of hydroxyethylstarch (HES) in haemodilution therapy of humans. In skin sections from patients who had received HES for otological reasons HES deposition was found by immunohistochemical methods in keratinocytes, epidermal dendritic cells, endothelial cells and macrophages. The aim of the present investigation was to study the interaction of cultured human monocytes and keratinocytes, normal (HNK) and malignant (KB, A431), with FITC-conjugated HES (200/0.5). Peripheral blood monocytes from healthy donors were isolated by Ficoll-Hypaque gradient centrifugation and adherence to plastic surfaces. Cells were incubated with HES-FITC (1 mg/ml) for 10, 20, 30, 60 and 120 min at 37
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01112917
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