ISSN:
1432-0983
Keywords:
Schizosaccharomyces pombe
;
Group I intron
;
Maturase
;
Recombinast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The open reading frame of the first intron of the mitochondrial cox1 gene (cox1I1) was expressed in Escherichia coli. The putative intron-encoded protein stimulated the formation of intra-chromosomal lac +-recombinants about threefold. No stimulation was found when the reading frame was inserted in the opposite direction, or when it was interrupted by a deletion. The intronic open reading frame did not complement recA − or recB − mutants of E. coli. In S. pombe, elimination of this intron did not abolish homologous recombination in mitochondria. A possible role of the recombinase activity in yeast mitochondria will be discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00355058
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