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  • Genomic probing  (1)
  • Key words In situ hybridization  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 778-786 
    Details
    ISSN: 1432-2242
    Keywords: Genomic probing ; In situ hybridization ; Interphase cytogenetics ; Physical mapping ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genomic in situ hybridization was used to identify alien chromatin in chromosome spreads of wheat, Triticum aestivum L., lines incorporating chromosomes from Leymus multicaulis (Kar. and Kir.) Tzvelev and Thinopyrum bessarabicum (Savul. and Rayss) Löve, and chromosome arms from Hordeum chilense Roem. and Schult, H. vulgare L. and Secale cereale L. Total genomic DNA from the introgressed alien species was used as a probe, together with excess amounts of unlabelled blocking DNA from wheat, for DNA:DNA in-situ hybridization. The method labelled the alien chromatin yellow-green, while the wheat chromosomes showed only the orange-red fluorescence of the DNA counterstain. Nuclei were screened from seedling root-tips (including those from half-grains) and anther wall tissue. The genomic probing method identified alien chromosomes and chromosome arms and allowed counting in nuclei at all stages of the cell cycle, so complete metaphases were not needed. At prophase or interphase, two labelled domains were visible in most nuclei from disomic lines, while only one labelled domain was visible in monosomic lines. At metaphase, direct visualization of the morphology of the alien chromosome or chromosome segment was possible and allowed identification of the relationship of the alien chromatin to the wheat chromosomes. The genomic in-situ hybridization method is fast, sensitive, accurate and informative. Hence it is likely to be of great value for both cytogenetic analysis and in plant breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227384
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  • 2
    Electronic Resource
    Electronic Resource
    Comparative physical mapping of the 5S and 18S-25S rDNA in nine wild Hordeum species and cytotypes (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1-9 
    Details
    ISSN: 1432-2242
    Keywords: Key words In situ hybridization ; Hordeum ; Karyotype evolution ; rDNA ; Barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Absract  The physical locations of the 5S and 18S-25S rDNA sequences were examined in nine wild Hordeum species and cytotypes by double-target in situ hybridization using digoxigenin-labelled 5S rDNA and biotin-labelled 18S-25S rDNA as probes. H. vulgare ssp. spontaneum (2n=2x=14; I-genome) had a similar composition of 5S and 18S-25S rDNA to cultivated barley (H. vulgare ssp. vulgare, I-genome), with two major 18S-25S rDNA sites and minor sites on four of the other five chromosomes; three chromosomes had 5S rDNA sites. The closely related H. bulbosum (2x; also I-genome) showed only one pair of 5S rDNA sites and one pair of 18S-25S rDNA sites on different chromosomes. Four wild diploid species, H. marinum (X-genome), H. glaucum and H. murinum (Y-genomes) and H. chilense (H-genome), differed in the number (2–3 pairs), location, and relative order of 5S and the one or two major 18S-25S rDNA sites, but no minor 18S-25S rDNA sites were observed. H. murinum 4x had three chromosome pairs carrying 5S rDNA, while the diploid had only a single pair. Two other tetraploid species, H. brachyantherum 4x and H. brevisubulatum 4x (both considered to have H-type genomes), had minor 18S-25S rDNA sites, as well as the major sites. Unusual double 5S rDNA sites – two sites on one chromosome arm separated by a short distance – were found in the American H-genome species, H. chilense and H. brachyantherum 4x. The results indicate that the species H. brachyantherum 4x and H. brevisubulatum 4x have a complex evolutionary history, probably involving the multiplication of minor rDNA sites (as in H. vulgare sensu lato), or the incorporation of both I and H types of genome. The rDNA markers are useful for an investigation of chromosome evolution and phylogeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051033
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    Paper (German National Licenses)
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