Note: Front Cover -- Applications of Chimeric Genes and Hybrid Proteins -- Copyright Page -- Table of Contents -- Contributors to Volume 327 -- Preface -- Volumes in Series -- Section I: Epitope Tags for Immunodetection -- Chapter 1. Epitope Tagging: General Method for Tracking Recombinant Proteins -- Section II: Markers for Cytology, Analysis of Protein Trafficking, and Lineage Tracing -- Chapter 2. Alkaline Phosphatase Fusions of Ligands or Receptors as in Situ Probes for Staining of Cells, Tissues, and Embryos -- Chapter 3. Chimeric Molecules Employing Horseradish Peroxidase as Reporter Enzyme for Protein Localization in the Electron Microscope -- Chapter 4. Biochemical Analyses of Trafficking with Horseradish Peroxidase-Tagged Chimeras -- Chapter 5. Visualizing Protein Dynamics in Yeast with Green Fluorescent Protein -- Chapter 6. Kinetic Analysis of Intracellular Trafficking in Single Living Cells with Vesicular Stomatitis Virus Protein G-Green Fluorescent Protein Hybrids -- Chapter 7. Dual Color Detection of Cyan and Yellow Derivatives of Green Fluorescent Protein Using Conventional Fluorescence Microscopy and 35-mm Photography -- Chapter 8. Invertase Fusion Proteins for Analysis of Protein Trafficking in Yeast -- Chapter 9. Introduction of Kex2 Cleavage Sites in Fusion Proteins for Monitoring Localization and Transport in Yeast Secretory Pathway -- Chapter 10. Lineage Analysis with Retroviral Vectors -- Chapter 11. Use of Pseudotyped Retroviruses in Zebrafish as Genetic Tags -- Section III: Tools for Analysis of Membrane Proteins -- Chapter 12. A Gene Fusion Method for Assaying Interactions of Protein Transmembrane Segments in Vivo -- Chapter 13. Using SUC2-HIS4C Reporter Domain to Study Topology of Membrane Proteins in Saccharomyces cerevisiae -- Chapter 14. Detecting Interactions between Membrane Proteins in Vivo Using Chimeras. , Chapter 15. Alkaline Phosphatase Fusion Proteins for Molecular Characterization and Cloning of Receptors and Their Ligands -- Chapter 16. Surface Chimeric Receptors as Tools in Study of Lymphocyte Activation -- Chapter 17. Use of Chimeric Receptor Molecules to Dissect Signal Transduction Mechanisms -- Chapter 18. Fusion Protein Toxins Based on Diphtheria Toxin: Selective Targeting of Growth Factor Receptors of Eukaryotic Cells -- Chapter 19. Green Fluorescent Protein-Based Sensors for Detecting Signal Transduction and Monitoring Ion Channel Function -- Chapter 20. Metabolic Labeling of Glycoproteins with Chemical Tags through Unnatural Sialic Acid Biosynthesis -- Section IV: Signals for Addressing Proteins to Specific Subcellular Compartments -- Chapter 21. Using Sorting Signals to Retain Proteins in Endoplasmic Reticulum -- Chapter 22. Directing Proteins to Nucleus by Fusion to Nuclear Localization Signal Tags -- Chapter 23. Identification, Analysis, and Use of Nuclear Export Signals in Saccharomyces cerevisiae -- Chapter 24. Directing Proteins to Mitochondria by Fusion to Mitochondrial Targeting Signals -- Chapter 25. Targeting Proteins to Plasma Membrane and Membrane Microdomains by N-Terminal Myristoylation and Palmitoylation -- Chapter 26. Analysis of Function and Regulation of Proteins That Mediate Signal Transduction by Use of Lipid-Modified Plasma Membrane-Targeting Sequences -- Chapter 27. Glycerolphosphoinositide Anchors for Membrane-Tethering Proteins -- Chapter 28. Fusions to Members of Fibroblast Growth Factor Gene Family to Study Nuclear Translocation and Nonclassic Exocytosis -- Section V: Application of Chimeras in Monitoring and Manipulating Cell Physiology -- Chapter 29. Posttranslational Regulation of Proteins by Fusions to Steroid-Binding Domains. , Chapter 30. Tet Repressor-Based System for Regulated Gene Expression in Eukaryotic Cells: Principles and Advances -- Chapter 31. Coumermycin-Induced Dimerization of GyrB-Containing Fusion Proteins -- Chapter 32. Use of Glutathione S-Transferase and Break Point Cluster Region Protein as Artificial Dimerization Domains to Activate Tyrosine Kinases -- Chapter 33. Recombinant Aequorin as Tool for Monitoring Calcium Concentration in Subcellular Compartments -- Chapter 34. Recombinant Aequorin as Reporter of Changes in Intracellular Calcium in Mammalian Cells -- Chapter 35. Monitoring Protein Conformations and Interactions by Fluorescence Resonance Energy Transfer between Mutants of Green Fluorescent Protein -- Chapter 36. Studies of Signal Transduction Events Using Chimeras to Green Fluorescent Protein -- Chapter 37. Use of Fusions to Green Fluorescent Protein in the Detection of Apoptosis -- Chapter 38. Synapto-pHluorins: Chimeras between pH-Sensitive Mutants of Green Fluorescent Protein and Synaptic Vesicle Membrane Proteins as Reporters of Neurotransmitter Release -- Chapter 39. Studying Organelle Physiology with Fusion Protein-Targeted Avidin and Fluorescent Biotin Conjugates -- Chapter 40. Fluorescent Labeling of Recombinant Proteins in Living Cells with FlAsH -- Chapter 41. Ubiquitin Fusion Technique and Its Descendants -- Chapter 42. Use of Phosphorylation Site Tags in Proteins -- Author Index -- Subject Index.

Note: Front Cover -- Aapplications of Chimeric Genes and Hybrid Proteins -- Copyright Page -- Table of Contents -- Contributors to Volume 326 -- Preface -- Volumes in Series -- Section I: Historical Overview -- Chapter 1. The All Purpose Gene Fusion -- Section II: Gene Fusions as Reporters of Gene Expression in Prokaryotic Cells -- Chapter 2. A Practical Guide to the Construction and Use of lac Fusions in Escherichia coli -- Chapter 3. Tagging Exported Proteins Using Escherichia coli Alkaline Phosphatase Gene Fusions -- Chapter 4. Applications of Gene Fusions to Green Fluorescent Protein and Flow Cytometry to the Study of Bacterial Gene Expression in Host Cells -- Chapter 5. IVET and RIVET: Use of Gene Fusions to Identify Bacterial Virulence Factors Specifically Induced in Host Tissues -- Chapter 6. Identification of Exported Bacterial Proteins via Gene Fusions to Yersinia pseudotuberculosis Invasin -- Section III: Gene Fusions as Reporters of Gene Expression in Eukaryotic Cells -- Chapter 7. Use of Imidazoleglycerolphosphate Dehydratase (His3) as a Biological Reporter in Yeast -- Chapter 8. Use of Fusions to Human Thymidine Kinase as Reporters of Gene Expression and Protein Stability in Saccharomyces cerevisiae -- Chapter 9. Use of Fusions to Thymidine Kinase -- Chapter 10. In Vivo Analysis of lacZ Fusion Genes in Transgenic Drosophila melanogaster -- Chapter 11. Utility of the Secreted Placental Alkaline Phosphatase Reporter Enzyme -- Chapter 12. Fusions to Imidazopyrazinone-Type Luciferases and Aequorin as Reporters -- Chapter 13. Novel Methods for Chemiluminescent Detection of Reporter Enzymes -- Chapter 14. Fusions to Chloramphenicol Acetyltransferase as a Reporter -- Chapter 15. Fusions to β-Lactamase as a Reporter for Gene Expression in Live Mammalian Cells -- Section IV: Functional Tags for Biochemical Purification. , Chapter 16. Purification of Proteins Using Polyhistidine Affinity Tags -- Chapter 17. Generating Fusions to Glutathione S-Transferase for Protein Studies -- Chapter 18. Use of the Strep-Tag and Streptavidin for Detection and Purification of Recombinant Proteins -- Chapter 19. Streptavidin-Containing Chimeric Proteins. Design and Production -- Chapter 20. Fusions to Maltose-Binding Protein: Control of Folding and Solubility in Protein Purification -- Chapter 21. Thioredoxin as a Fusion Partner for Production of Soluble Recombinant Proteins in Escherichia coli -- Chapter 22. Affinity Purification of Recombinant Proteins Fused to Calmodulin or to Calmodulin-Binding Peptides -- Chapter 23. The S · Tag Fusion System for Protein Purification -- Chapter 24. Fusions to Self-Splicing Inteins for Protein Purification -- Chapter 25. Fusion Proteins Containing Cellulose-Binding Domains -- Chapter 26. Biotinylation of Proteins in Vivo and in Vitro Using Small Peptide Tags -- Chapter 27. Biotinylation of Proteins in Vivo: A Useful Posttranslational Modification for Protein Analysis -- Section V: Hybrid Proteins for Detection and Production of Antigens and Antibodies -- Chapter 28. Methods for Generating Multivalent and Bispecific Antibody Fragments -- Chapter 29. Design and Use of Phage Display Libraries for the Selection of Antibodies and Enzymes -- Chapter 30. Use of an Lpp-OmpA Fusion Vehicle for Bacterial Surface Display -- Chapter 31. Identification of Bacterial Class I Accessible Proteins by Disseminated Insertion of Class I Epitopes -- Chapter 32. Bordetella pertussis Adenylate Cyclase Toxin: A Vehicle to Deliver CD8-Positive T-Cell Epitopes into Antigen-Presenting Cells -- Chapter 33. Use of Anthrax Toxin Fusions to Stimulate Immune Responses -- Chapter 34. Use of Fusions to Viral Coat Proteins as Antigenic Carriers for Vaccine Development -- Author Index. , Subject Index.

Note: Front Cover -- Applications of Chimeric Genes and Hybrid Proteins -- Copyright Page -- Table of Contents -- Contributors to Volume 328 -- Preface -- Volume in Series -- Section I: Applications of Two-Hybrid Methods and Related Techniques for Analysis of Protein-Protein Interactions -- Chapter 1. High-Throughput Screening for Protein-Protein Interactions Using Two-Hybrid Assay -- Chapter 2. LexA-Based Two-Hybrid Systems -- Chapter 3. Interaction Mating Methods in Two-Hybrid Systems -- Chapter 4. Analysis and Identification of Protein-Protein Interactions Using Protein Recruitment Systems -- Chapter 5. A Bacterial Two-Hybrid System that Exploits a cAMP Signaling Cascade in Escherichia coli -- Chapter 6. A Green Fluorescent Protein-Based Reverse Two- Hybrid System: Application to the Characterization of Large Numbers of Potential Protein- Protein Interactions -- Chapter 7. Yeast Three-Hybrid System for Detecting Ligand Receptor Interactions -- Chapter 8. Use of a Yeast Three-Hybrid System to Clone Bridging Proteins -- Chapter 9. The Yeast Tribrid System: cDNA Expression Cloning of Protein Interactions Dependent on Posttranslational Modifications -- Chapter 10. The Yeast Split-Hybrid System -- Section II: Other Approaches Using Chimeras for Identification and Analysis of Interactions of Proteins and Nucleic Acids -- Chapter 11. Mapping Protein-Protein Interaction Domains Using Ordered Fragment Ladder Far-Western Analysis of Hexahistidine-Tagged Fusion Proteins -- Chapter 12. Mapping Specificity Determinants for Protein- Protein Association Using Protein Fusions and Random Peptide Libraries -- Chapter 13. Selection of Genetic Agents from Random Peptide Aptamer Expression Libraries -- Chapter 14. Detection of Protein-Protein Interactions by Protein Fragment Complementation Strategies. , Chapter 15. Monitoring Protein-Protein Interactions in Live Mammalian Cells by B-Galactosidase Complementation -- Chapter 16. Green Fluorescent Protein Chimeras to Probe Protein-Protein Interactions -- Chapter 17. Protein Fusions to Coiled-Coil Domains -- Chapter 18. Molecular Applications of Fusions to Leucine Zippers -- Chapter 19. Using the Yeast Three-Hybrid System to Detect and Analyze RNA-Protein Interactions -- Chapter 20. Principles and Applications of a Tat-Based Assay for Analyzing Specific RNA-Protein Interactions in Mammalian Cells -- Section III: Phage Display and Its Applications -- Chapter 21. Phage Display for Selection of Novel Binding Peptides -- Chapter 22. Selectively Infective Phage Technology -- Chapter 23. Use of Phage Display and Transition-State Analogs to Select Enzyme Variants with Altered Catalytic Properties: Glutathione Transferase as an Example -- Chapter 24. Selecting and Evolving Functional Proteins in Vitro by Ribosome Display -- Chapter 25. Yeast Surface Display for Directed Evolution of Protein Expression, Affinity, and Stability -- Section IV: Construction of Hybrid Molecules by DNA Shuffiing and Other Methods -- Chapter 26. Methods for in Vitro DNA Recombination Random Chimeragenesis -- Chapter 27. Random Chimeragenesis by Heteroduplex Recombination -- Chapter 28. Use of Chimeras Generated by DNA Shuffiing: Probing Structure-Function Relationships among Glutathione Transferases -- Chapter 29. Protein Engineering by Expressed Protein Ligation -- Section V: Applications of Fusions in Functional Genomics -- Chapter 30. A Systematic and General Proteolytic Method for De.ning Structural and Functional Domains of Proteins -- Chapter 31. High-Throughput Expression of Fusion Proteins -- Chapter 32. Rapid Construction of Recombinant DNA by the Univector Plasmid-Fusion System. , Chapter 33. High-Throughput Methods for the Large-Scale Analysis of Gene Function by Transposon Tagging -- Chapter 34. GATEWAY Recombinational Cloning: Application to the Cloning of Large Numbers of Open Reading Frames or ORFeomes -- Chapter 35. Gene Trapping Methods for the Identification and Functional Analysis of Cell Surface Proteins in Mice -- Author Index -- Subject Index.