ISSN:
1432-0983
Keywords:
Yeast
;
β-glucanase
;
Bacillus
;
Gene expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The endo-β-1,3-1,4-glucanase gene from B. subtilis was placed under yeast promoter control in a number of different yeast expression vectors. The hybrid plasmids were transformed into S. cerevisiae where they directed the synthesis of varying amounts of active enzyme. The presence of B. subtilis DNA sequences 5′ to the initiation codon for the B. subtilis β-glucanase gene reduced expression of the gene in yeast. A 1,000-fold increase in the yield of β-glucanase was obtained using the ADH1 promoter compared with the CYC1 promoter.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00389427
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