ISSN:
1432-0983
Keywords:
Volvox carteri
;
Actin gene
;
Introns
;
Expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Southern blot analysis of Volvox carteri DNA indicated the presence of a single actin gene; the nucleotide sequence of that gene is reported here. In comparison with plant animal and fungal actins, the derived primary structure of 377 amino acids is highly conserved yielding similarity values of 79% to 94% (including nonidentical, conservative exchanges). In contrast, the intron structure of the gene is highly unusual: in addition to one intron in the 5′ untranslated region (ten nucleotides upstream of the initiator ATG), it has eight introns in the coding region, only three of which are in locations where introns have previously been reported. Transcription starts 26 nucleotides downstream of the putative TATA box and 70 nucleotides downstream of a conspicuous CCAAT motif. A potential polyadenylation signal, TGTAA, is located 366 nucleotides downstream of the terminator TAA. Northern hybridization indicates that the actin gene is transcribed throughout the Volvox life cycle with only a slight depression during the release of juveniles from mother spheroids. This pattern of gene expression suggests that actin may assume various functional roles in the differentiation and growth of Volvox.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00318215
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