ISSN:
1432-203X
Keywords:
Key wordsAcampe praemorsa
;
Epiphytic orchid
;
In vitro plant regeneration
;
Thidiazuron
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An efficient and reproducible procedure is described for the large-scale propagation of an epiphytic orchid, Acampe praemorsa (Roxb.) Blatter and McCann using foliar explants. Shoot buds were induced in basal parts of foliar explants on Murashige and Skoog medium supplemented with N6-benzyladenine (BA), kinetin (Kn) or thidiazuron (TDZ), the latter being most effective at 1.0 mg/l. Shoots formed to a TDZ-containing medium elongated following transfer to a substrate supplemented with 2.0 mg/l 1-naphthaleneacetic acid (NAA) and 0.5 mg/l BA. NAA at lower concentrations had no beneficial effects on shoot regeneration, whether added to the medium along with BA, Kn or TDZ. However, it promoted shoot elongation and leaf expansion. Higher concentrations of NAA suppressed shoot regeneration. The frequency of shoot regeneration was greatly influenced by the developmental stage and orientation of the leaf. Shoots regenerated from the foliar explants were rooted successfully on MS medium containing 1.0 mg/l indole-3-butyric acid. The plantlets were acclimated and eventually transferred to a garden.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01142328
Permalink
