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  • 1
    Online Resource
    Online Resource
    Oxford :Oxford University Press, Incorporated,
    Keywords: Sustainable development. ; Environmental policy. ; Environmental protection. ; Electronic books.
    Description / Table of Contents: Shocks, States, and Sustainability outlines a theory for when we can expect long-term changes toward sustainability. Thomas K. Rudel offers historical comparisons of radical reforms in environmental practices to show that societies become more sustainable in the aftermath of earth-shaking events events that underline the limits of our natural resources.
    Type of Medium: Online Resource
    Pages: 1 online resource (233 pages)
    Edition: 1st ed.
    ISBN: 9780190924461
    DDC: 333.7
    Language: English
    Note: Cover -- Shocks, States, and Sustainability -- Copyright -- Dedication -- Contents -- Preface -- Acknowledgments -- Chapter 1. Introduction -- Chapter 2. Radical Environmental Reforms: A Theory -- Chapter 3. The Great Plains: Soil Conservation During the "Dirty Thirties" -- Chapter 4. England: Green Belts After World War II -- Chapter 5. Cuba: Agro-​Ecological Farming After the Soviet Collapse -- Chapter 6. Coastal Maine: A Catch-​and-​Sometimes-​Release Lobster Fishery -- Chapter 7. The World: Reform in a Global Environmental Cage -- Chapter 8. Radical Environmental Reforms in Comparative Perspective -- Chapter 9. Conclusion -- Notes -- References -- Index.
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  • 2
    ISSN: 1617-4623
    Keywords: Key words Plasmid vector ; Conjugation ; Generalized mutagenesis ; Homologous recombination ; Natural transformation competence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A versatile shuttle system has been developed for genetic complementation with cloned genes of transformable and non-transformable Neisseria mutants. By random insertion of a selectable marker into the conjugative Neisseria plasmid ptetM25.2, a site within this plasmid was identified that is compatible with plasmid replication and with conjugative transfer of plasmid. Regions flanking the permissive insertion site of ptetM25.2 were cloned in Escherichia coli and served as a basis for the construction of the Hermes vectors. Hermes vectors are composed of an E. coli replicon that does not support autonomous replication in Neisseria, e.g. ColE1, p15A, or ori fd, fused with a shuttle consisting of a selectable marker and a multiple cloning site flanked by the integration region of ptetM25.2. Complementation of a non-transformable Neisseria strain involves a three-step process: (i) insertion of the desired gene into a Hermes vector; (ii) transformation of Hermes into a Neisseria strain containing ptetM25.2 to create a hybrid ptetM25.2 via gene replacement by the Hermes shuttle cassette; and (iii) conjugative transfer of the hybrid ptetM25.2 into the final Neisseria recipient. Several applications for the genetic manipulation of pathogenic Neisseriae are described.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1617-4623
    Keywords: Plasmid vector ; Conjugation ; Generalized mutagenesis ; Homologous recombination ; Natural transformation competence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A versatile shuttle system has been developed for genetic complementation with cloned genes of transformable and non-transformableNeisseria mutants. By random insertion of a selectable marker into the conjugativeNeisseria plasmidptetM25.2, a site within this plasmid was identified that is compatible with plasmid replication and with conjugative transfer of plasmid. Regions flanking the permissive insertion site of ptetM25.2 were cloned inEscherichia coli and served as a basis for the construction of the Hermes vectors. Hermes vectors are composed of anE. coli replicon that does not support autonomous replication inNeisseria, e.g. ColE1, p15A, orori fd, fused with a shuttle consisting of a selectable marker and a multiple cloning site flanked by the integration region of ptetM25.2. Complementation of a non-transformableNeisseria strain involves a three-step process: (i) insertion of the desired gene into a Hermes vector; (ii) transformation of Hermes into aNeisseria strain containing ptetM25.2 to create a hybrid ptetM25.2 via gene replacement by the Hermes shuttle cassette; and (iii) conjugative transfer of the hybrid ptetM25.2 into the finalNeisseria recipient. Several applications for the genetic manipulation of pathogenicNeisseriae are described.
    Type of Medium: Electronic Resource
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