ISSN:
1572-8773
Keywords:
Enterobactin
;
Dihydroxybenzoylserine
;
Isolation
;
HPLC-separation
;
Ion spray mass spectrometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary The production of catecholate siderophores was studied in some selected species ofEnterobacter (Enterobacteriaceae). The extracted catecholates were separated as iron-free compounds by HPLC on a C18 reversed-phase column using methanol/0.1% phosphoric acid or methanol/0.1% formic acid as a solvent system and identified by ion spray mass spectrometry (LC/MS, MS/MS). Five catecholate compounds were identified which include 2,3-dihydroxybenzoylserine, its linear dimer and trimer, the cyclic enterobactin and an unidentified isomer of enterobactin. In addition, a new large-scale method for the isolation of catecholate siderphores is described which is based on adsorption on XAD-2 and subsequent purification on Sephadex LH20.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01135388
Permalink