Keywords:
Regenerative medicine.
;
Electronic books.
Description / Table of Contents:
This book shows that different kinds of signal - chemical, topographical and electrical - can be arranged in a highly controlled way. Using unconventional fabrication techniques, the text uses scale lengths ranging from several micrometers to a few nanometers.
Type of Medium:
Online Resource
Pages:
1 online resource (102 pages)
Edition:
1st ed.
ISBN:
9783642228810
Series Statement:
Springer Theses Series
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=798733
DDC:
571.6
Language:
English
Note:
Intro -- Multiscale Fabrication of Functional Materials for Regenerative Medicine -- Supervisor's Foreword -- Preface -- Acknowledgments -- Contents -- 1 Introduction -- 1.1…The Age of Nanotechnologies -- 1.2…Nanofabrication -- 1.3…Regenerative Medicine and CNS Diseases -- References -- 2 Experimental Techniques -- 2.1…Fabrication Techniques -- 2.1.1 Photolithography -- 2.1.1.1 The Photoresist -- 2.1.1.2 Pre-Baking -- 2.1.1.3 Mask Alignment -- 2.1.1.4 Development and Post-Baking -- 2.1.2 Soft-Lithography -- 2.1.3 Replica Molding -- 2.1.4 MicroMolding in Capillaries -- 2.1.5 Lithographically Controlled Wetting -- 2.2…Characterization Techniques -- 2.2.1 Atomic Force Microscopy -- 2.2.1.1 Working Principle -- 2.2.1.2 The Cantilever -- 2.2.1.3 Operating Modes -- 2.2.2 Scanning Electron Microscopy -- 2.2.2.1 Working Principle -- 2.2.2.2 Sample Coating -- 2.2.3 Optical Microscopy -- 2.2.4 X-Ray Photoelectron Spectroscopy -- References -- 3 Stable Non-Covalent Functionalization of Teflon-AF -- 3.1…Introduction -- 3.2…Laminin Functionalization of Teflon-AF -- 3.3…Laminin Immunofluorescence Assay -- 3.4…Neural Cell Growth on Laminin-Patterned Teflon-AF -- 3.5…Materials and Methods -- 3.5.1 Preparation of Telfon-AF Films -- 3.5.2 Pattern Fabrication on PTFE Surface -- 3.5.3 Characterization of the Patterned Substrates -- 3.5.4 Testing of the Cell Adhesion and Growth -- 3.6…Conclusions -- References -- 4 Stamp-Assisted Multiscale Patterning of TiO2 for Cell Growth Control -- 4.1…Introduction -- 4.2…Fabrication of Porous and Flat TiO2 Patterns -- 4.3…Neural Cell Adhesion and Proliferation on TiO2 Patterns -- 4.4…Neural Cell Density Dependence on Stripe Width -- 4.5…Materials and Methods -- 4.5.1 Colloidal Composite Preparation -- 4.5.2 Titanium Dioxide Pattern Fabrication -- 4.5.3 Titanium Dioxide Pattern Characterization -- 4.5.4 Neural Cell Cultures.
,
4.5.5 Neural Cell Adhesion and Proliferation Assays -- 4.5.6 Neural Cell Viability Assay -- 4.5.7 Cytoskeleton and Focal Adhesion Staining by Immunofluorescence Assay -- 4.5.8 Cell Morphology Analysis by Combined SEM, AFM and Optical Microscopies -- 4.5.9 Data Analysis -- 4.6…Conclusions -- References -- 5 Control of Neural Cell Adhesion on 3D-SWCNT -- 5.1…Introduction -- 5.2…Neural Cell Adhesion on SWCNT Pattern -- 5.2.1 Patterning of SWCNT by GAD -- 5.2.2 Cell Adhesion on SWCNT Array -- 5.3…Electric Stimulation of Cells on SWCNT-Based Device -- 5.3.1 Test Bed Fabrication -- 5.3.2 Electric Stimulation -- 5.4…Materials and Methods -- 5.4.1 SWCNT Suspension in O-benzyl Tyrosine and SWCNT/PS Composite -- 5.4.1.1 Patterning of SWCNT by GAD -- 5.4.1.2 Cell Adhesion on SWCNT Arrays -- 5.4.1.3 Fabrication of Test Bed -- 5.4.1.4 Electric stimulation -- 5.4.1.5 Statistical Analysis -- 5.5…Conclusions -- References -- 6 Lithographically Controlled Etching -- 6.1…Introduction -- 6.2…Surface Patterning by Lithographically Controlled Etching -- 6.3…Simulation by Finite Elements of the Lithographically Controlled Etching Process -- 6.4…SiO2 Nanowire Fabrication -- 6.5…One-Pot Surface Patterning and Nanoparticle Deposition -- 6.6…X-Ray Photoelectron Spectroscopy -- 6.7…Absorption Spectroscopy -- 6.8…Materials and Methods -- 6.8.1 Substrate, Stamps and Solutions -- 6.8.2 Atomic Force Microscopy -- 6.8.3 X-Ray Photoelectron Spectroscopy -- 6.9…Conclusions -- 6.10…Outlook of Lithographically Controlled Etching -- References -- 7 Conclusions.
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