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  • 1
    Online Resource
    Online Resource
    Toronto :University of Toronto Press,
    Keywords: Electronic books.
    Description / Table of Contents: This book should be of particular interest to all who, through the nature of their research, are required to maintain cultures of micro-organisms and other cells in a healthy and stable state. It is based on papers and discussion at the Specialists' Conference on Culture Collections, Ottawa, Canada, August, 1962.
    Type of Medium: Online Resource
    Pages: 1 online resource (237 pages)
    Edition: 1st ed.
    ISBN: 9781487582715
    Series Statement: Heritage Series
    Language: English
    Note: Cover -- Preface -- Future conferences -- Welcome to delegates - W. H. Cook -- Chairman's remarks - N. E. Gibbons -- Role and organization of culture collections -- Introductory remarks - R. E. Buchanan -- Culture collection -- why and wherefore - A. L . van Beverwijk -- Discussion I - S. T. Cowan -- Discussion II - R. Donovick -- General discussion -- The organization of a type culture collection - J. M. Shewan -- Discussion I - W. A. Clark -- Discussion II - W. C. Haynes -- General discussion -- Fundamental aspects of cell preservation -- Introductory remarks - T. O. Wikén -- Loss of adaptive enzyme during storage - S. G. Bradley -- Discussion I - D. H. Braendle -- Discussion II - W. E. Brown -- General discussion -- Mechanisms of injury in frozen and frozen-dried cells - P. Mazur -- Discussion I - R. J. Heckly, R. L. Dimmick and J. J. Windle -- Discussion II - T. Nei -- General discussion -- Assessment of methods of preservation -- General methods for preserving cultures - K. B. Raper -- Discussion I - N. A. Krasilnikov -- Discussion II - H. Proom -- General discussion -- Fungus cultures: conservation and taxonomic responsibility - E. G. Simmons -- Discussion I - B. L. Brady -- Discussion II - E. O. Stapley -- General discussion -- Maintenance of strains of Bacillus species - R. E. Gordon and T.K. Rynearson -- Discussion I - H. P. R. Seeliger -- Discussion II - M. P. Starr -- General discussion -- Culture collections of algae - R. C. Starr -- Discussion I - E. A. George -- Discussion II - N. D. Levine -- General discussion -- Preservation of viruses and bacteriophage - R. E. O. Williams and E. A. Asheshov -- Discussion I - R. L. Thompson -- Discussion II - R. Wahl -- General discussion -- Preservation and characterization of animal cell strains - C. S. Stulberg -- Discussion I - J. F. Morgan -- Discussion II - R. E. Stevenson. , General discussion -- Submitted papers -- Estimation of storage life of liquid and dry stock cultures of Pasteurella tularensis and of spores of Bacillus anthracis - I. A. Dearmon, M. D. Orlando, A. J. Rosenwald, F. Klein, A. L. Fernelius, R. E. Lincoln and P. R. Middaugh -- Technological solutions to problems in preservation at cryogenic temperatures - C. W. Cowley and A. P. Rinfret -- Fundamentals in the application of cryogenic temperatures to the maintenance of viability in micro-organisms - S. W. Moline, A. W. Rowe, G. F. Doebbler and A. P. Rinfret -- Preservation of parasitic protozoa in liquid nitrogen - L. S. Diamond, H. T. Meryman and E. Kafig -- The effect of some treatments for starvation upon the viability of yeast cells through lyophilization - G. Terui and J. Ikeda -- Quantitative study of damage and survival of bacteria during storage over a ten year period - A. P. Harrison and M. J. Pelczar -- The storage of mycobacteria in the dried state: the effect of the culture medium - P. W. Muggleton -- The influence of freeze-drying on the metabolism of mycobacteria - R. Bonicke.
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  • 2
    Online Resource
    Online Resource
    Berlin, Heidelberg :Springer Berlin / Heidelberg,
    Keywords: Cellular signal transduction. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (234 pages)
    Edition: 1st ed.
    ISBN: 9783540450351
    Series Statement: Topics in Current Chemistry Series ; v.211
    DDC: 571.7/36
    Language: English
    Note: 211 Topics in Current Chemistry -- Bioorganic Chemistry of Biological Signal Transduction -- Copyright -- Preface -- Contents -- Protein Tyrosine Kinase Inhibitors as Therapeutic Agents -- Peptidomimetic SH2 Domain Antagonists for Targeting Signal Transduction -- Biophysical Characterization of the Ras Protein -- Ras-Farnesyltransferase-Inhibitors as Promising Anti-Tumor Drugs -- Phosphatidylcholine-Preferring Phospholipase C from B. cereus. Function, Structure, and Mechanism -- Photoaffinity Labeling in Biological Signal Transduction -- Author Index Volume 201-211.
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  • 3
    Online Resource
    Online Resource
    San Diego :Elsevier Science & Technology,
    Keywords: Measles virus. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (260 pages)
    Edition: 1st ed.
    ISBN: 9781483272146
    DDC: 616.9/15/0194
    Language: English
    Note: Front Cover -- Measles Virus and its Biology -- Copyright Page -- Table of Contents -- PREFACE -- Chapter 1. The biology of measles -- THE EPIDEMIOLOGY OF MEASLES -- THE PATHOGENESIS OF MEASLES -- COMMENT -- Chapter 2. The isolation and adaptation of measles virus -- ISOLATION OF MEASLES VIRUS -- ADAPTATION TO TISSUE CULTURE AND CHICK EMBRYO -- ADAPTATION TO MAMMALIAN HOSTS -- SSPE MEASLES VIRUSES -- COMMENT -- Chapter 3. Biological characters and genetic markers -- HOST RANGE -- CYTOPATHIC EFFECT -- PATHOGENICITY AND VIRULENCE IN ANIMALS -- PLAQUE FORMATION -- HAEMAGGLUTINATION -- TEMPERATURE-SENSITIVE MUTANTS -- SSPE STRAINS -- PHYSICAL PROPERTIES -- MEASLES, CANINE DISTEMPER AND RINDERPEST VIRUSES -- COMMENT -- Chapter 4. Structure and components of measles virus -- THE VIRION -- NUCLEOCAPSID -- VIRION RNA -- STRUCTURAL POLYPEPTIDES -- COMMENT -- Chapter 5. Non-vegetative functions of measles virus, haemagglutination, haemolysis and cell-fusion -- HAEMAGGLUTINATION -- HAEMOLYSIS -- CELL FUSION FROM WITHOUT THE CELL -- COMMENT -- Chapter 6. The growth cycle of measles virus -- GENERAL FEATURES -- THE ASSAY -- ADSORPTION -- PENETRATION -- THE REPLICATIVE PROCESSES -- MATURATION AND RELEASE OF MEASLES VIRUS -- MORPHOGENESIS OF MEASLES VIRUS -- Chapter 7. Persistent infection by measles virus -- BIOLOGICAL ASPECTS OF PERSISTENT INFECTION -- MOLECULAR ASPECTS OF PERSISTENT INFECTION -- GENESIS OF PERSISTENT INFECTION -- COMMENT-THE FEATURES OF PERSISTENT INFECTION -- Chapter 8. Neuropathogenicity of measles virus -- EXPERIMENTAL ENCEPHALITIS -- THE EFFECT OF AGE OF THE HOST ON SUSCEPTIBILITY -- PATHOGENESIS OF ENCEPHALITIS -- SIGNIFICANCE OF FINDINGS -- Chapter 9. Measles virus and subacute sclerosing panencephalitis- SSPE-viruses -- ASSOCIATION BETWEEN MEASLES VIRUS AND SSPE -- HOST RANGE AND VARIABILITY OF ISOLATES -- SYNTHESIS OF VIRUS ANTIGEN. , BIOCHEMICAL STUDIES ON SSPE VIRUSES -- NEUROPATHOGENICITY -- COMMENT -- Chapter 10. Immunology of measles virus-epidemiology -- specific antibodies -- EPIDEMIOLOGY AND IMMUNITY -- TESTS FOR ANTIBODY -- APPEARANCE AND DURATION OF CIRCULATING ANTIBODY -- CLASSES OF SPECIFIC IMMUNOGLOBULIN -- ANTIBODY AND INFECTED CELLS -- COMMENT -- Chapter 11. Immunology of measles virus-cell-mediated immunity -- CIRCULATING LEUKOCYTES AND MEASLES VIRUS -- NON-SPECIFIC RESPONSES FOLLOWING MEASLES IN VIVO -- NON-SPECIFIC RESPONSES FOLLOWING INFECTION IN VITRO -- SPECIFIC RESPONSES TO MEASLES ANTIGEN -- COMMENT -- Chapter 12. Immunology of measles virus-multiple sclerosis and auto-immune diseases -- MULTIPLE SCLEROSIS -- SYSTEMIC LUPUS ERYTHEMATOSIS AND AUTO-IMMUNE STATES -- COMMENT -- Chapter 13. Immunology of measles virus-immunization -- immunodeficient states -- IMMUNIZATION -- IMMUNODEFICIENCY AND IMMUNOSUPPRESSION -- COMMENT -- References -- Subject Index.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 21 (1992), S. 15-24 
    ISSN: 0886-1544
    Keywords: actin edge-bundle ; cortical tension ; cell shape ; microfilaments ; cell adhesion ; cell motility ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have previously described actin edge-bundles (AEBs) as cables of microfil-aments lining the webbed edges of 3T3 cells (Zand and Albrecht-Buehler: Cell Motil. Cytoskeleton 13:195-211, 1989). We have suggested that AEBs, along with their cell-substratum adhesions, resist cortical tension and prevent the collapse of cytoplasm towards the nucleus. In this paper, we report several stages of AEB disassembly and re-formation induced by the following micro-manipulations(1)Scoring of the webbed edge of a 3T3 cells with a microneedle. As a result the sides of the score retracted and the severed AEB appeared to disassemble down to its terminal adhesion points. The retraction stopped after 20-40 seconds and the cells formed a webbed edge with large curvature. Over a period of 20-80 minutes, the new web decreased in length and depth, until it regained its approximate original shape.(2)Bending of cell processes at acute angles. As a result the processes moved until they projected at right angles to the side of the cell and formed new webs gradually expanded their area. In both cases, the nascent webs were lined by actin edge-bundles.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 13 (1989), S. 195-211 
    ISSN: 0886-1544
    Keywords: cell shape ; cortical actin ; stress fibers ; microfilament bundles ; cell adhesion ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The outline of cells in sparse cultures consists prediminantly of concave and convex segments; straight segments are rare and ephemeral. The convex segments are areas of active cell expansion. The concave segments are stationary and web-shaped, similar in profile to the cables of a suspension bridge. In 3T3 fibroblasts, we have found a single microfilament bundle following the outline of every webbed edge and have called it the actin edge-bundle (AEB). While the AEB is composed predominantly of actin, α-actinin and myosin are also present. In contrast to normal stress fibers, AEBs are more resistant to several treatments that depolymerize F-actin. Once an AEB disassembles, however, the webbed edge collapses and retracts, suggesting that the actin edge-bundle is a specialized cytoskeletal structure that supports the webbed edges of interphase 3T3 fibroblasts. The stability of AEBs is independent of microtubules. We suggest that the microfilament bundles that frequently line the lateral contacts between epithelial cells in vivo may be related to the actin edge-bundle.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 13 (1989), S. 94-103 
    ISSN: 0886-1544
    Keywords: cell adhesion ; cell motility ; near infrared light ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Interference-reflection microscopy (IRM) is the only method presently available with which to visualize cell-substratum adhesions in living tissue culture cells continuously for long periods of time without the use of fluorescent markers (Curtis: J. Cell Biol. 20:199-215, 1964; Izzard and Lochner: J. Cell Sci. 21:129-159, 1976). This method utilizes approximately 1% of the incident illumination to produce the IRM image (Verschueren: J. Cell Sci. 75:279-301, 1985) and so far has required the use of high-intensity light sources in the visible spectral range (400-800 nm). Unfortunately, visible light of this intensity and spectral range induces marked changes in the behavior and morphology of motile fibroblasts, including cessation of locomotion. In contrast, the present paper reports that continuous observations of live cells in IRM for periods of up to 8 hours are possible if the illuminating light is in the red to near-infrared range (650-950 nm) and without any observable change in normal cell morphology or behavior. In addition, we describe how the technique of Y-contrast image processing can be applied to IRM images to create a three-dimensional image of the ventral cell surface topography.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 46 (1991), S. 115-124 
    ISSN: 0730-2312
    Keywords: basement membrane ; laminin ; oligosaccharides ; cell spreading ; neurite outgrowth ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We have examined how laminin carbohydrates participate in cellular responses and have focused upon cell spreading and neurite outgrowth. Our earlier studies showed that unglycosylated laminin fully supported cell adhesion but did not promote subsequent spreading of mouse melanoma cells or neurite outgrowth of rat pheochromocytoma cells (Dean et al. (1990): J Biol Chem 265:12553-12562). In the present experiments, we determined whether those cellular responses could be restored to adherent cells. When a mixture of unglycosylated and glycosylated laminins was used as a substratum for mouse melanoma cells, some cells began to spread when 30% glycosylated laminin was present. At least 65% glycosylated laminin was required to elicit a maximal spreading response by the majority of the cells. In separate experiments, we found that cell spreading was fully restored by a pronase digest of glycosylated laminin; a similar digest of unglycosylated laminin had no effect. These results indicate that laminin carbohydrates, rather than polypeptide sequences, were responsible for cell spreading. We also conclude that substrate attachment of the carbohydrate moieties was not essential. In other experiments, laminins containing immature oligosaccharides were produced using two glycosylation pathway inhibitors, swainsonine or castanospermine. When such laminins were used to study cell spreading or neurite outgrowth, laminin containing immature oligosaccharides was as effective as laminin which contains fully processed oligosaccharides. In contrast, laminin with partially processed oligosaccharides had incomplete activity. These composite reconstitution experiments show that laminin carbohydrates provide essential information to responsive cells, enabling them to progress from an adherent state to a spread form or to extend neurite processes.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 90 (1977), S. 9-14 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The way in which the lectins concanavalin A (Con A) and Ricinus communis agglutinin (Ricin) alter the K+ content of Ehrlich ascites tumor cells was investigated. Unidirectional and net fluxes were determined in unwashed cells during a time course following lectin addition. Total influx, ouabain sensitive influx, Mg++- and Na+-K+-ATPase activity were all unaffected. Cell ATP content was normal for at least 19 minutes after exposure to Con A. Early after contact with Ricin or Con A efflux was stimulated 2-3-fold, resulting in net K+ loss, but after 20 minutes efflux had returned to normal. Ricin and Con A acted similarly although Ricin was present at only 1/50 the concentration of Con A. When the findings are evaluated together with previous work it is suggested that a particular membrane glycoprotein may be concerned in the efflux alteration observed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 83 (1974), S. 243-250 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Net fluxes of sodium and potassium were studied in Ehrlich mouse ascites tumor cells during contact with the agglutinating protein, concanavalin A. This lectin altered cation transport markedly at concentrations of 20-105 μg/ml (6-47 μg/mg cell protein). Whereas control cells extruded sodium and maintained or accumulated potassium against electrochemical gradients, in the presence of concanavalin A there was rapid net sodium entry and potassium loss. After 10-20 minutes in concanavalin A, sodium extrusion began and potassium loss diminished but these events were prevented by ouabain. The alterations in cation content induced by concanavalin A are unlikely to be the result only of agglutination since soybean agglutinin caused much smaller changes although it agglutinated the cells equally well.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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