ISSN:
1573-0603
Keywords:
Haematopoietic tissue
;
Nephrops norvegicus
;
Ovary
;
Primary cell cultures
;
Testes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract This paper reports the first attempt to develop primary cell cultures from tissues (ovary, testes, hepatopancreas, haematopoietic tissue, heart, gut, gill, eye-stalk and nerve tissues) of the Dublin Bay prawn (also known as the Norwegian squat lobster and scampi) Nephrops norvegicus (L.) using Leibovitz's L-15, MEM and M199 media supplemented with 5, 10 or 20 percent (w/v) foetal bovine serum (FBS). The best results were achieved with ovary tissues in 2× Leibovitz's L-15. Also, primary cell cultures were developed with testicular and haematopoietic tissues. With ovary tissues, results with 5 percent (w/v) FBS at an osmolality of 800 mOsm/kg were superior for the maintenance of epithelial-type cells, and enabled longer survival, i.e. more than 3 months. Little success was achieved with M199 and MEM media. One subculture of ovary cells was obtained.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009787223797
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