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  • ELISA  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Antigenic diversity of cytochromes c3 from the anaerobic, sulfate-reducing bacteria,Desulfovibrio (1984)
    Springer
    Archives of microbiology 139 (1984), S. 91-94 
    Details
    ISSN: 1432-072X
    Keywords: Cytochrome c3 ; Desulfovibrio ; Sulfate-reducing bacteria ; Enzyme-linked immunoadsorption assay ; ELISA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An indirect enzyme-linked immunoadsorption assay (ELISA) was developed for cytochrome c3 using antisera to the cytochromes fromDesulfovibrio africanus Benghazi, Desulfovibrio vulgaris Hildenborough andDesulfovibrio salexigens British Guiana. The ELISA system was used to test for cross-reactions between these antisera and the heterologous antigens. In contrast to previous experiments using the Ouchterlony technique, all of the cytochromes c3 tested exhibited some degree of cross-reaction. Considerable variation was seen in cross-reactions for cytochromes c3 from differing strains ofD. desulfuricans. This observation raises questions about the taxonomic relatedness of these strains. No cross-reaction was seen with eukaryotic cytochrome c or withD. vulgaris cytochrome c553. The data demonstrate that cytochrome c3 is capable of undergoing nonprecipitating cross-reactions, and thus may not be as immunologically unique as was once thought.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692719
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Whole-cell antigens of members of the sulfate-reducing genus Desulfovibrio (1985)
    Springer
    Archives of microbiology 141 (1985), S. 195-197 
    Details
    ISSN: 1432-072X
    Keywords: Desulfovibrio ; Sulfate-reducing bacteria ; Enzyme-linked-immunoadsorption assay ; ELISA ; Whole-cell antigens ; Cytochrome c3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Antisera have been developed against the wholecell antigens of Desulfovibrio africanus Benghazi and Walvis Bay, D. vulgaris Hildenborough, D. salexigens British Guiana, D. gigas, and D. desulfuricans Essex 6. An enzymelinked immunoadsorption assay (ELISA) was developed to measure the reaction of these antisera with the homologous and heterologous antigens. The ELISA method demonstrated a reaction between pre-immune sera and cells of D. africanus, D. gigas and D. desulfuricans, suggesting the presence of a lectin-like substance on these cell surfaces. Extensive cross-reactions were seen between the antisera and heterologous cells, suggesting the sharing of a number of surface antigens amongst the Desulfovibrio. However, the pattern of these cross-reactions was different from that observed for an ELISA reaction developed for the cytochrome c3 from various Desulfovibrio.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408057
    Location Call Number Limitation Availability
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    Paper (German National Licenses)
    Fulltext
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