ISSN:
1432-0878
Keywords:
Neurosecretion
;
Sinus gland
;
Isolated nerve endings
;
Potassium stimulation
;
Digitonin permeabilization
;
Cardisoma carnifex
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The preparation of isolated peptidergic nerve terminals from the sinus gland (a neurohemal organ) of the crab (Cardisoma carnifex) is described. In this species the nerve endings can have diameters up to 30 μm. They release neurosecretory material as judged by the decrease in the volumetric density of granules upon depolarization with potassium. Similar results were obtained after permeabilization of the nerve terminals with digitonin, but only in the presence of micromolar concentrations of calcium. This preparation should prove useful in correlating electrical events with other cellular processes involved in stimulus-secretion coupling.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00215899
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