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  • 1
    Electronic Resource
    Electronic Resource
    Power and limits of DNA-profiling in primate populations: Paternity assessment in rhesus macaques from Cayo Santiago (1993)
    Springer
    Primates 34 (1993), S. 395-402 
    Details
    ISSN: 0032-8332
    Keywords: DNA fingerprinting ; Oligonucleotides ; Paternity assessment ; Heterozygosity ; Rhesus monkeys
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Paternity testing was performed in one social group (S) of rhesus macaques from Cayo Santiago, Puerto Rico. In 11/15 cases, sires could be identified comparing the multilocus DNA profiles of 19 males to those of the corresponding mother/child dyads. All 19 males could be excluded from paternity in the remaining four cases. Decision making was partly based on likelihoods of DNA profiles, and the theoretical model underlying these calculation is described. In a second social group (M), held in captivity, paternity testing was impeded by a deficit of maternal bands and by an increased extent of band sharing of mothers and their infants. Some possible explanations for these findings, including increased homozygosity in group M, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02382635
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  • 2
    Electronic Resource
    Electronic Resource
    Male rank, reproductive behavior, and reproductive success in free-ranging rhesus macaques (1993)
    Springer
    Primates 34 (1993), S. 481-489 
    Details
    ISSN: 0032-8332
    Keywords: Macaca mulatta ; Cayo Santiago ; DNA fingerprinting ; Paternity ; Mating success ; Dominance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Paternity assessment through DNA fingerprinting by synthetic oligonucleotide probes was applied to one birth cohort in a social group of free-ranging rhesus macaques (Macaca mulatta) on Cayo Santiago. The 11 group males and 9 males from other groups were observed mating with the females. Paternity was determined for 11 of the 15 infants. Male dominance rank was not associated with reproductive success. High-ranking resident males (N=5) sired 27% of the infants born during a one-year study. Four of the 11 infants of known paternity were sired by males of other social groups. The four infants of unknown paternity were sired either by males not observed mating with the females or the low-ranking male who was not fingerprinted. Male dominance rank was not associated with reproductive activity during conception cycles. These results suggest that the effect of rank on male reproductive success is not a predictable correlation, but a conditional probability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02382659
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  • 3
    Electronic Resource
    Electronic Resource
    A novel standardization method for two-dimensional DNA fingerprints (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 2874-2879 
    Details
    ISSN: 0173-0835
    Keywords: DNA fingerprinting ; Two-dimensional DNA electrophoresis ; Numerical iteration ; Relaxation method ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Two-dimensional (2-D) DNA fingerprinting is a technique that allows for parallel genome analysis through the simultaneous detection of up to 500 minior microsatellite loci on a 2-D gel. Separation is performed according to size and melting temperature in the gel. In the application of this technique in genome analysis, a standardized method for the identification of individual spots is required. However, due to the polymorphic nature of up to 80% of the spots, existing standardization methods that have been primarily developed for 2-D protein patterns are not suitable for this task. We developed a robust method that standardizes 2-D DNA fingerprint spots on the basis of melting temperature - or denaturing gradient position - and fragment size. An external marker was used as a basis for standardization. A normalization surface was calculated over the gel dimensions by adapting an established numerical iteration technique previously used in physics termed “relaxation method”. The relaxation method works robustly with the irregularly spaced marker spots. The evaluation of the method for a spot of preknown position derived from the TP53 gene revealed a median observed error below 1% for fragment length and denaturing gradient position. The search for candidate minisatellite loci in genomic difference analysis depends on the reliable identification of alleles of this locus in different individuals. We proved experimentally that alleles of a single minisatellite locus cloned from a 2-D gel cluster on an isothermal line can be reliably identified using the presented standardization method. In conclusion, a standardization tool for a broader application of 2-D DNA fingerprinting in both tumor analysis and possibly parallel mutation screening is now available.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150181527
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  • 4
    Electronic Resource
    Electronic Resource
    Parallel genome analysis by one- and two-dimensional DNA fingerprinting in human gliomas (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1715-1725 
    Details
    ISSN: 0173-0835
    Keywords: DNA fingerprinting ; Two-dimensional DNA typing ; Genome scanning ; Tumor analysis ; Gliomas ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The detection of DNA variation in cancers is an important step in elucidating the mechanism of tumorigenesis. Using the strategy of multipoint genome analysis we detected many differences between glioma-derived and constitutional DNA by customary DNA fingerprinting with simple repetitive oligonucleotide probes. Amplification of the epidermal growth factor receptor (EGFR) gene has been found to be easily detectable as new or highly intensified bands in one-dimensional (1-D) DNA fingerprints of glioblastoma DNA generated with probes (GTG)5 or (GT)8. However, in most low-grade astrocytomas, 1-D DNA fingerprinting has failed to reveal any genomic abnormalities. In these cases a two-dimensional (2-D) technique was successfully employed that is based on size separation in neutral gels followed by sequence-dependent separation in denaturing gradient gels and hybridization with several mini- and microsatellite core probes. The hundreds of spots visualized with this technique were used to detect subtle changes probably occurring as the initial steps of tumorigenesis in human gliomas. On average, five of the approximately 580 sports generated by probes CAC and 33.6 were found to be altered in tumor DNA; 80% of the alterations were spot losses, the rest being spot gains or amplifications. Computer-based image analysis using an external lambda marker provided a stringent way to compare spot patterns generated by 2-D DNA finger-printing. In comparisons performed between typing patterns generated on the same gel, 99% of truly identical spots were confirmed by the sofware. In intergel comparisons 84% of identical spots were matched on the basis of the marker information alone.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601284
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