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  • Crucifereae  (1)
  • Isotope  (1)
  • Key words In situ hybridization  (1)
  • Tandemly repeated DNA sequence  (1)
Document type
Keywords
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    The long-term excretion and retention of an intravenous dose of45Ca in two healthy men (1973)
    Springer
    Calcified tissue international 12 (1973), S. 217-226 
    Details
    ISSN: 1432-0827
    Keywords: Calcium ; Isotope ; Radiobiology ; Excretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé La rétention du45Ca a été déterminée pendant 388 jours après une injection intraveineuse en mesurant les excrétions urinaires et fécales et en tenant compte des pertes par la peau. Chez les deux hommes sains étudiés, les taux d'excrétion urinaire sont semblables, mais les taux d'excrétion fécale diffèrent par un facteur de deux. La rétention, exprimée en dose moin excrétion, suit une loi de puissance au bout des 4 ou 7 premières semaines mais, à une période plus précoce, un facteur exponentiel surajouté est nécessaire pour rendre compte des résultats. Les concentrations plasmatiques ont été mesurées jusqu'à quinze jours après l'injection. Les clearances rénales des deux sujets ne sont pas significativement différentes.
    Abstract: Zusammenfassung Die Retention von45Ca wurde 388 Tage lang nach einer intravenösen Injektion bestimmt, indem Urin- und Stuhlausscheidungen unter Berücksichtigung des Verlustes durch die Haut gemessen wurden. Bei den zwei untersuchten gesunden Männern war die Geschwindigkeit der Ausscheidung im Urin sehr ähnlich, die Stuhlausscheidungsraten unterschieden sich jedoch durch einen Faktor von zwei. Die Retention, die als Injektionsdosis minus Ausscheidung ausgedrückt wurde, folgte nach den ersten 4 oder 7 Wochen einer Exponentialfunktion, vor diesem Zeitpunkt war aber noch ein zusätzliches Exponentialglied notwendig, um die Daten auszudrücken. Die entsprechenden Plasmakonzentrationen wurden 15 Tage lang nach der Injektion gemessen. Die Nieren-Clearance beider Männer waren nicht signifikant verschieden.
    Notes: Abstract The retention of45Ca has been determined up to 388 days after an intravenous injection by measuring the urinary and faecal excretions and correcting for the loss from the skin. In the two healthy men examined, the urinary excretion rates were very similar but the faecal excretion rates differed by a factor of two. Retention expressed as dose minus excretion followed a power law after the first 4 or 7 weeks but at earlier times an additional exponential term was necessary to account for the data. Plasma concentrations of the dose were measured up to 15 days after the injection. The renal clearances for the two subjects were not significantly different.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02013736
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  • 2
    Electronic Resource
    Electronic Resource
    The distribution, organization and evolution of two abundant and widespread repetitive DNA sequences in the genus Hordeum (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 169-176 
    Details
    ISSN: 1432-2242
    Keywords: Key words Barley ; Hordeum ; In situ hybridization ; Phylogeny ; Tandemly repeated DNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The genomic organization and chromosomal distributions of two abundant tandemly repeated DNA sequences, dpTa1 and pSc119.2, were examined in six wild Hordeum taxa, representing the four basic genomes of the genus, by Southern and fluorescence in situ hybridization. The dpTa1 probe hybridized to between 30 and 60 sites on the chromosomes of all five diploid species studied, but hybridization patterns differed among the species. Hybridization of the pSc119.2 sequence to the chromosomes and Southern blots of digested DNA detected signals in Hordeum bulbosum, Hordeum chilense, Hordeum marinum and Hordeum murinum 4x, but not in Hordeum murinum 2x and Hordeum vulgare ssp. spontaneum. A maximum of one pSc119.2 signal was observed in the terminal or subterminal region of each chromosome arm in the species carrying this sequence. The species carrying the same I-genome differed in the presence (Hordeum bulbosum) or absence (Hordeum spontaneum) of pSc119.2. The presence of pSc119.2 in the tetraploid cytotype of Hordeum murinum, but its absence in the diploid cytotype, suggests that the tetraploid is not likely to be a simple autotetraploid of the diploid. Data about the inter- and intra-specific variation of the two independent repetitive DNA sequences give information about both the interrelationships of the species and the evolution of the repetitive sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050023
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  • 3
    Electronic Resource
    Electronic Resource
    Comparative physical mapping of the 5S and 18S-25S rDNA in nine wild Hordeum species and cytotypes (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1-9 
    Details
    ISSN: 1432-2242
    Keywords: Key words In situ hybridization ; Hordeum ; Karyotype evolution ; rDNA ; Barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Absract  The physical locations of the 5S and 18S-25S rDNA sequences were examined in nine wild Hordeum species and cytotypes by double-target in situ hybridization using digoxigenin-labelled 5S rDNA and biotin-labelled 18S-25S rDNA as probes. H. vulgare ssp. spontaneum (2n=2x=14; I-genome) had a similar composition of 5S and 18S-25S rDNA to cultivated barley (H. vulgare ssp. vulgare, I-genome), with two major 18S-25S rDNA sites and minor sites on four of the other five chromosomes; three chromosomes had 5S rDNA sites. The closely related H. bulbosum (2x; also I-genome) showed only one pair of 5S rDNA sites and one pair of 18S-25S rDNA sites on different chromosomes. Four wild diploid species, H. marinum (X-genome), H. glaucum and H. murinum (Y-genomes) and H. chilense (H-genome), differed in the number (2–3 pairs), location, and relative order of 5S and the one or two major 18S-25S rDNA sites, but no minor 18S-25S rDNA sites were observed. H. murinum 4x had three chromosome pairs carrying 5S rDNA, while the diploid had only a single pair. Two other tetraploid species, H. brachyantherum 4x and H. brevisubulatum 4x (both considered to have H-type genomes), had minor 18S-25S rDNA sites, as well as the major sites. Unusual double 5S rDNA sites – two sites on one chromosome arm separated by a short distance – were found in the American H-genome species, H. chilense and H. brachyantherum 4x. The results indicate that the species H. brachyantherum 4x and H. brevisubulatum 4x have a complex evolutionary history, probably involving the multiplication of minor rDNA sites (as in H. vulgare sensu lato), or the incorporation of both I and H types of genome. The rDNA markers are useful for an investigation of chromosome evolution and phylogeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051033
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  • 4
    Electronic Resource
    Electronic Resource
    Centromeric repetitive DNA sequences in the genus Brassica (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 157-165 
    Details
    ISSN: 1432-2242
    Keywords: Maps (genetic) ; Nuclear architecture Repetitive DNA ; Ribosomal DNA ; Satellite DNA Brassica ; Crucifereae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Representatives of two major repetitive DNA sequence families from the diploid Brassica species B. campestris and B. oleracea were isolated, sequenced and localized to chromosomes by in situ hybridization. Both sequences were located near the centromeres of many chromosome pairs in both diploid species, but major sites of the two probes were all on different chromosome pairs. Such chromosome specificity is unusual for plant paracentromeric repetitive DNA. Reduction of stringency of hybridization gave centromeric hybridization sites on more chromosomes, indicating that there are divergent sequences present on other chromosomes. In tetraploid species derived from the diploids, the number of hybridization sites was different from the sum of the diploid ancestors, and some chromosomes had both sequences, indicating relatively rapid homogenization and copy number evolution since the origin of the tetraploid species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222197
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