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  • Clonality  (1)
  • Ground-penetrating radar  (1)
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  • 1
    ISSN: 1432-2307
    Keywords: Key words Composite lymphoma ; Lymphoplasmacytoid non-Hodgkin’s lymphoma ; Spleen ; Clonality ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We describe here the first well-characterized case of ”composite” lymphoma of the spleen in which the two components were a low-grade and a high-grade B-cell non-Hodgkin’s lymphomas. The patient was an elderly man with prominent splenomegaly and multiple hypoechogenic lesions of the spleen. A splenectomy was performed, and the macroscopic and histological findings showed the simultaneous presence of a ”low-grade” B-cell lymphoma, lymphoplasmacytoid (immunocytoma) and a ”high-grade” B-cell lymphoma (immunoblastic), which were spatially separated. The two lesions expressed the same immunoglobulin light chain (lambda), but the Southern blot analysis showed different patterns of immunoglobulin heavy chain (IgH) clonal rearrangement. PCR analysis followed by direct sequencing of the IgH-amplified rearrangement products provided molecular-genetic evidence that the two components of the composite lymphoma had the same clonal origin. Since both EBV LMP-1 and p53 were negative by immunohistochemistry, it is unlikely that EBV and p53 were involved in the neoplastic progression in this case. PCR analysis and direct sequencing of IgH-amplified rearrangement products are useful tools to investigate clonality in cases in which Southern blot analysis cannot be performed or does not provide conclusive findings.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2018-03-30
    Description: The results of a Ground-penetrating radar (GPR) survey were compared with the archaeological excavation outcomes of a twelfth century abbey site (Badia Pozzeveri). The goal was to associate the types of reflections recorded in GPR profiles and high-amplitude features visible in amplitude maps with unearthed archaeological features. GPR profiles crossing the walls evidenced axes of hyperbolic point source reflections and short planar reflections respectively generated by the stones forming the lateral sides/upper corners and the top of the structure. Moreover, the stones in the core of the wall caused small hyperbolic point source reflections with interfering axes, which produced a chaotic reflection profile. The resampling and gridding of these reflections collectively generated high-amplitude linear features in the amplitude maps. The presence in the graves of bones generates small hyperbolic point source reflections with interfering axes in the GPR profile, with a consequently chaotic reflection profile. Moreover, the existence of lythic slabs topping and/or siding the graves generates short planar reflections and axes of hyperbolic point source reflections. The resampling and gridding of these types of reflections generates in the amplitude map features that cannot be locally distinguished from those caused by the local aggregation of sediments with no archaeological relevance. The GPR profiles crossing trenches and pit-kiln showed dipping reflectors unconformably resting on a basal planar reflector. Disconformity marks the onset of trench and pit-kiln decommissioning with the infilling of clayey silty layers and anthropogenic remains. These features in the amplitude maps correspond to medium to high-amplitude areas. The results show that types of reflection can be associated to specific elements of archaeological structure for a detailed interpretation of a complex subsurface setting. Furthermore, it is evidenced that the interpretation of GPR profiles must be considered an indispensable pre-requisite for a full comprehension of amplitude maps
    Description: CA.RI.LU grant (2012-13): “New high resolution Georadar techniques for archeological contexts analysis” (Leader. A. Ribolini).
    Description: Published
    Description: 141 – 156
    Description: 7T. Struttura della Terra e geodinamica
    Description: JCR Journal
    Keywords: Ground-penetrating radar ; archaeological stratigraphy
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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