ISSN:
1432-072X
Keywords:
Methylobacillus flagellatum
;
Obligate methylotroph
;
Inc P1 plasmids
;
Retrotransfer
;
Chromosome mapping
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Homologous matings with plasmids R68.45 and pULB113, and also with Hfr type donor were employed for mapping pgi and gpd genes involved in C-1 metabolism in the obligate methylotroph Methylobacillus flagellatum. A preliminary map of the late chromosomal region was constructed on the basis of these experimental results. The C-1 markers were linked to methionine and leucine auxotrophy and nalidixic acid resistance markers. The phenomenon of retrotransfer, or shuttle transfer of chromosomal markers by Inc P1 plasmids, revealed earlier, was demonstrated for M. flagellatum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00247811
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