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  • Chloroplast DNA  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Chloroplast DNA variation in European potato cultivars (1990)
    Springer
    Potato research 33 (1990), S. 505-513 
    Details
    ISSN: 1871-4528
    Keywords: Reciprocal differences ; Chloroplast DNA ; Andigena ; Neo-tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The chloroplast DNA of 27 potato cultivars and 2 wild species has been examined by Southern blotting using probes representing the entire chloroplast genome of barley. 16% of the probe × enzyme combinations detected polymorphism between the potato cultivars. Cvs Maris Piper, Croft, Shelagh, Stormont Enterprise and Estima and the wild species could be distinguished from the other cultivars by their chloroplast DNA restriction patterns. These results are discussed in relation to cytoplasmic diversity and potato improvement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358029
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Chloroplast DNA diversity in the genusRubus (Rosaceae) revealed by Southern hybridization (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 65-75 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rosaceae ; Rubus ; Chloroplast DNA ; restriction fragment length polymorphism ; cladistic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The variability in chloroplast DNA type of 20Rubus genotypes was examined by Southern hybridization. DNA extracted from theRubus accessions was digested with two restriction enzymes (EcoRI and EcoRV) and heterologous chloroplast DNA sequences from barley and pea were used as probes to detectRubus chloroplast DNA sequences on Southern blots ofRubus total DNA. Restriction fragment length polymorphism was detected and a total of 92 restriction fragments were generated by the probe/enzyme combinations examined. Cladistic principles based on the parsimony assumption were used to assemble a phylogenetic tree based on chloroplast restriction fragment length data. The phylogenetic tree grouped the taxonomically defined species and is in general agreement with information based on morphological criteria. However, the Japanese red raspberryR. illecebrosus was shown to have diverged considerably in terms of evolutionary time from other species in subg.Idaeobatus. Furthermore, the molecular approach provides a quantitative estimate of the relationship between species that is difficult to obtain from morphological data. In order to complement the chloroplast DNA information a ribosomal DNA probe was also included in the analysis and provided further information on the phylogenetic relationships withinRubus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937798
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    Paper (German National Licenses)
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