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  • Solanum tuberosum  (3)
  • Chloroplast DNA  (2)
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Keywords
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Potato research 33 (1990), S. 505-513 
    ISSN: 1871-4528
    Keywords: Reciprocal differences ; Chloroplast DNA ; Andigena ; Neo-tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The chloroplast DNA of 27 potato cultivars and 2 wild species has been examined by Southern blotting using probes representing the entire chloroplast genome of barley. 16% of the probe × enzyme combinations detected polymorphism between the potato cultivars. Cvs Maris Piper, Croft, Shelagh, Stormont Enterprise and Estima and the wild species could be distinguished from the other cultivars by their chloroplast DNA restriction patterns. These results are discussed in relation to cytoplasmic diversity and potato improvement.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1871-4528
    Keywords: Solanum tuberosum ; abscisic acid ; mannitol ; N-dimethyl-succinamic acid ; nodal segments ; osmotic stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Cultures of 23 potato cultivars were examined for their responses to hormonal and osmotic growth inhibitors incorporated in media used for storage in-vitro. Cultivars differed in their tolerance of inhibitors and also in their growth on a Murashige and Skoog medium with 3% sucrose. Both osmotic and hormonal growth retardants restricted the development of shoot tips but there were significant cultivar x treatment interactions, indicating that the ranking of cultivars will vary with the media used. Such interactions may complicate the establishment of facilities for storage in-vitro. However, addition to the basal medium of 5 mg l−1 abscisic acid reduced both the overall mean scores and, more importantly, the between cultivar variance; its use in conjunction with low temperature shows promise as a method for conserving potato germplasm in-vitro.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 172 (1990), S. 65-75 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Rosaceae ; Rubus ; Chloroplast DNA ; restriction fragment length polymorphism ; cladistic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The variability in chloroplast DNA type of 20Rubus genotypes was examined by Southern hybridization. DNA extracted from theRubus accessions was digested with two restriction enzymes (EcoRI and EcoRV) and heterologous chloroplast DNA sequences from barley and pea were used as probes to detectRubus chloroplast DNA sequences on Southern blots ofRubus total DNA. Restriction fragment length polymorphism was detected and a total of 92 restriction fragments were generated by the probe/enzyme combinations examined. Cladistic principles based on the parsimony assumption were used to assemble a phylogenetic tree based on chloroplast restriction fragment length data. The phylogenetic tree grouped the taxonomically defined species and is in general agreement with information based on morphological criteria. However, the Japanese red raspberryR. illecebrosus was shown to have diverged considerably in terms of evolutionary time from other species in subg.Idaeobatus. Furthermore, the molecular approach provides a quantitative estimate of the relationship between species that is difficult to obtain from morphological data. In order to complement the chloroplast DNA information a ribosomal DNA probe was also included in the analysis and provided further information on the phylogenetic relationships withinRubus.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 23 (1990), S. 159-164 
    ISSN: 1573-5044
    Keywords: frequency distribution ; haploids ; multiplicative binomial ; poisson ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methods for the production and utilisation of haploids have been developed for a range of crop plants. Genetical and environmental factors are known to influence both the rate of haploid induction and the mode of regeneration. Investigations designed to examine the parameters that influence haploid production from anthers are usually based on the overall percentage of responding anthers. Previous studies have rarely taken into account the frequency or distribution of anther culture response within a potato flower. In this paper the binomial, poisson and multiplicative binomial distributions are used for the first time to describe the distribution of anther culture response within a Solanum tuberosum flower.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 23 (1990), S. 181-186 
    ISSN: 1573-5044
    Keywords: heritability ; protoplasts ; RFLPs ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genetics of tissue culture response in potato has been examined by analysing a sample of dihaploids (2n=2x=24) extracted from tetraploid parents (4n=4x=48). The genotypes were screened for rate of nodal multiplication, in vitro tuberisation, regeneration from leaf discs and protoplast plating efficiency. Significant differences were detected between dihaploids for the traits measured and this indicates that tissue culture response in the tetraploid parents must be in the heterozygous condition. Estimates of the broad sense heritabilities were calculated together with the number of genes or effective factors involved in the control of the traits. These estimates indicate that tissue culture response in potato is under relatively simple genetic control and “blocks of genes” may be located on specific chromosomes. The inheritance of RFLP markers in the segregating dihaploid population was also monitored and the potential of using molecular markers linked to gene(s) controlling tissue culture response is discussed.
    Type of Medium: Electronic Resource
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