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  • 1
    Electronic Resource
    Electronic Resource
    Relationship between interferon therapy and variability in nonstructural gene 5b of hepatitis C virus (1998)
    Springer
    Journal of gastroenterology 33 (1998), S. 684-693 
    Details
    ISSN: 1435-5922
    Keywords: Key words: hepatitis C virus ; nonstructural gene 5 ; quasispecies ; sequence analysis ; interferon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: The hepatitis C virus (HCV) quasispecies nature in the hypervariable region (HVR) has been reported and found to relate to the effectiveness of interferon (IFN) treatment. However, the quasispecies nature in the nonstructural (NS) 5b region remains to be addressed. To examine this characterization and relationship with IFN therapy, we sequenced six independent HCV clones from each of eight patients. The eight patients were classified as responders or nonresponders to IFN. In the four responders, we found one to three isolates in each of the six clones. In the nonresponders, the six clones consisted of four, five, six, and six isolates, respectively. Compared the (NS) 5b genes of the isolates obtained from the patients with that of the reported hepatitis C virus HC-C2 or HC-J6 isolate the ratio of nonsynonymous to total substitutions ranged from 17.61% to 30.95% in the responders and from 33.11% to 76.47% in the nonresponders. We also compared posttreatment with pretreatment sequences. The average number of varying amino acids ranged from 5.5 to 9.0 in isolates remaining after IFN treatment and from 4.3 to 5.5 in the isolates that disappeared with IFN treatment. Two changed amino acids (glycine to arginine and valine to isoleucine) (compared with the pretreatment clones) were found in the posttreatment clones of one of the responders and one amino acid change (valine to alanine) was found in another responder. These results suggest that the NS5b quasispecies correlates with IFN treatment effectiveness. These results also implied that the heterogeneity in different hierarchical strata has a common impact on IFN treatment, making infected patients resistant to IFN. Our study also provides evidence that HCV elimination and mutation may occur simultaneously during IFN therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005350050156
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The influence of the p53 gene on the in vitro chemosensitivity of colorectal cancer cells (1999)
    Springer
    Journal of cancer research and clinical oncology 125 (1999), S. 357-360 
    Details
    ISSN: 1432-1335
    Keywords: Key words Colorectal neoplasms ; Apoptosis ; Chemosensitivity ; p53 gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose : The p53 gene is considered one of the most important in the control of apoptosis, and its mutations have a close relationship with chemosensitivity. The aim of this work was to investigate the role of p53 in the apoptosis of colorectal cancer cells in vitro, induced by 5-fluorouracil (5-FU) and hydroxy-camptothecin (HCPT). Methods : A total of 39 colorectal cancer samples from patients were treated in vitro with 5-FU (10 μg/ml), 5-FU (10 μg/ml) + leucovorin (5 μg/ml), HCPT (0.1 μg/ml) and HCPT (0.1 μg/ml) + Salvia mitorrhiza (6 μl), using an in situ terminal deoxynucleotidyltransferase assay to detect chemosensitivity. p53 gene mutations from tumor DNA were detected, after amplification by the polymerase chain reaction of exons 5–8, by non-radioactive single-strand conformation polymorphism. Results : p53 gene mutations were observed in 43.6% (17/39) of colorectal carcinomas, when the terminal deoxynucleotidyltransferase assay was used to detect the tumor apoptotic rate. Cells with mutated p53 had lower chemosensitivity than those without (p 〈 0.01). Conclusion : Routine assessment of p53 status may be helpful in selecting patients with the wild-type p53 gene, who have a predictably better response to chemotherapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004320050286
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic variability and characterization of non-structural region 5 of hepatitis C virus genome from Chinese patients (1998)
    Springer
    Journal of gastroenterology 33 (1998), S. 62-72 
    Details
    ISSN: 1435-5922
    Keywords: Key words: hepatitis C virus ; nonstructural region 5 ; sequence analysis ; China
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: Sequence variation in the putative nonstructural region 5b (NS5b) of hepatitis C virus (HCV) was analyzed in China. Complementary DNA fragments from sera of 49 Chinese patients were amplified by polymerase chain reaction (PCR) and the products were cloned and sequenced. Based on the comparison in NS5b of 33 clones of genotype 1b and 16 clones of genotype 2a, Chinese isolates of HCV belong to the same subtype as HCV-J, and HC-J6 from Japan. There does exist, however, some heterogeneity in the primary structure of the nucleotide acid. Higher homology was found among Chinese isolates than among Chinese isolates and Japanese isolates. Furthermore, among Chinese isolates, we found some conserved nucleotide acid positions different from those of Japanese isolates. Comparison of average homology among the 33 clones of genotype 1b and the 16 clones of genotype 2a indicated that the average homology among genotype 2a was lower than that among genotype 1b. In addition, a deletion of three nucleotide acids and a frame-shift, resulting in the introduction of an in-frame stop codon, were first observed in the NS5b region. These results indicated geographical differences in the distribution of individual HCV isolates, and the existence of a local variant in the same subtype. Our findings also suggested the need for further study on the sequence of genotype 2a, to improve diagnosis and help to advance the development of a vaccine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005350050046
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  • 4
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    Electronic structure of the ingredient planes of the cuprate superconductor Bi_{2} Sr_{2} CuO_{6+δ} : A comparison study with Bi_{2} Sr_{2} CaCu_{2} O_{8+δ} (2016)
    American Physical Society (APS)
    Details
    Publication Date: 2016-04-17
    Description: Author(s): Yan-Feng Lv, Wen-Lin Wang, Hao Ding, Yang Wang, Ying Ding, Ruidan Zhong, John Schneeloch, G. D. Gu, Lili Wang, Ke He, Shuai-Hua Ji, Lin Zhao, Xing-Jiang Zhou, Can-Li Song, Xu-Cun Ma, and Qi-Kun Xue By means of low-temperature scanning tunneling microscopy, we report on the electronic structures of the BiO and SrO planes of the Bi 2 Sr 2 CuO 6 + δ (Bi-2201) superconductor prepared by argon-ion bombardment and annealing. Depending on post annealing conditions, the BiO planes exhibit either a pseudogap … [Phys. Rev. B 93, 140504(R)] Published Fri Apr 15, 2016
    Keywords: Superfluidity and superconductivity
    Print ISSN: 1098-0121
    Electronic ISSN: 1095-3795
    Topics: Physics
    Published by American Physical Society (APS)
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  • 5
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    Coupled dipole oscillations of a mass-imbalanced Bose-Fermi superfluid mixture (2018)
    American Physical Society (APS)
    Details
    Publication Date: 2018-01-23
    Description: Author(s): Yu-Ping Wu, Xing-Can Yao, Xiang-Pei Liu, Xiao-Qiong Wang, Yu-Xuan Wang, Hao-Ze Chen, Youjin Deng, Yu-Ao Chen, and Jian-Wei Pan Recent experimental realizations of superfluid mixtures of Bose and Fermi quantum gases provide a unique platform for exploring diverse superfluid phenomena. We study dipole oscillations of a double superfluid in a cigar-shaped optical dipole trap, consisting of K 41 and Li 6 atoms with a large mass i... [Phys. Rev. B 97, 020506(R)] Published Mon Jan 22, 2018
    Keywords: Superfluidity and superconductivity
    Print ISSN: 1098-0121
    Electronic ISSN: 1095-3795
    Topics: Physics
    Published by American Physical Society (APS)
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