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  • Phosphatidylinositol  (2)
  • Cell culture (cytokinin and cell division)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Planta 169 (1986), S. 216-221 
    ISSN: 1432-2048
    Keywords: Callus ; 1,2-Diacylglycerol ; Glycine (phosphatidylinositol) ; Inositol phosphates ; Phosphatidylinositol ; Phospholipase C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have investigated the breakdown of membrane-bound phosphatidylinositol (PI) in homogenates of soybean (Glycine max) callus. The breakdown of PI was stimulated by the detergent deoxycholate. At pH 7.0 and 1·gl-1 of deoxycholate the loss of PI was rapid and extensive: more than 80% was broken down within 10 min. The breakdown of PI was also stimulated by millimolar concentrations of Ca2+. The products of breakdown of added PI (purified from soybean callus) in this system were identified from their chromatographic mobilities as 1,2-diacylglycerol, myo-inositol 1-phosphate and myo-inositol 1:2-cyclic monophosphate.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Cell culture (cytokinin and cell division) ; Cell division ; Cytokinin and cell division ; Glycine (cell culture) ; Phosphatidylinositol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is described for preparing fully viable, cytokinin-starved soybean (Glycine max (L.) Merr. cv. Acme) cells from a suspension-culture of callus tissue. The cells respond to kinetin treatment by re-initiating cell division. We present evidence, from the pattern of incorporation of 32P-labelled inorganic phosphate into individual phospholipids during the first hour of this response, that the synthesis of phosphatidylinositol (PI) and of phosphatidic-acid head-groups is affected within 15 min. The polyphosphoinositide phosphatidylinositol 4-phosphate, but not phosphatidylinositol 4,5-bisphosphate, was detected in the tissue. The characteristics of cytokinin-induced PI synthesis in cytokinin-starved soybean cells appear to resemble the ‘PI response’ of animal cells.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
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