ISSN:
0730-2312
Schlagwort(e):
very low density lipoprotein
;
hen
;
oocyte membranes
;
Life and Medical Sciences
;
Cell & Developmental Biology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Chemie und Pharmazie
,
Medizin
Notizen:
The interaction of hen 125I-VLDL (very low density lipoprotein) with chicken oocyte membranes was characterized using a rapid sedimentation assay. Equilibrium and kinetic studies showed an apparent dissociation constant (Kd) 8.7-9.1 × 10-8 M or 43.5-45.5 μg VLDL protein/ml. Binding capacity was 2.0 μg VLDL protein/mg membrane homogenate protein. The apparent rate constants were, k1 = 2.4 × l05 M-1min-1 and k2 = 2.1 × 10-2 min-1. Specific binding required the presence of divalent cations. Whereas binding was completely re-stored after treatment with EDTA by the addition of Mn++, only 60% of binding was restored using Ca++.
Zusätzliches Material:
3 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jcb.240280406
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