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  • 1
    Electronic Resource
    Electronic Resource
    Visualization of neuropeptide-binding sites on individual telencephalic neurons of the rat (1993)
    Springer
    Cell & tissue research 272 (1993), S. 523-531 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin ; Cholecystokinin ; Binding sites ; Receptors ; Neurons ; Telencephalon ; Rat (Han: WIST)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The identification of high-affinity binding sites for neuropeptides on individual target cells is a prerequisite when studying the sites of action and the manner in which peptides act as neuromediators. In situ and in vitro, this can be achieved using newly synthesized, biologically active conjugates of somatostatin or cholecystokinin (sulphated octapeptide) with colloidal gold. Labelled neurons show a peptide-specific, non-overlapping distribution in rat telencephalic structures; i.e, whereas the somatostatin-gold conjugate labels binding sites on neurons and glial cells, cholecystokinin-binding sites are restricted to neurons. Binding of either gold-labelled ligand can be competitively suppressed by excess amounts of the native peptide or its analogues. Neuronal somatostatin-binding sites are visualized on neurons in lamina III and, in particular, in lamina V/VI of the primary somatosensory cortex and in the magnocellular nucleus of the telencephalic cholinergic system. Cholecystokinin-binding sites are localized in the main olfactory bulb, on neurons in the cortical “hindlimb” and “forelimb” region, in the hippocampus, and in the cingulate and visual cortex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318559
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The ultrastructural basis of steroid production in the Y-organ and the mandibular organ of the crabs Hemigrapsus nudus (Dana) and Carcinus maenas L. (1980)
    Springer
    Cell & tissue research 206 (1980), S. 83-94 
    Details
    ISSN: 1432-0878
    Keywords: Carcinus ; Ecdysone ; Hemigrapsus ; Mandibular organ ; Y-organ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the steroid producing Y-organ and the mandibular organ of the crustaceans Hemigrapsus nudus and Carcinus maenas has been studied with reference to the well investigated steroid secreting cells (SSC) of mammals. In accordance with the most important characteristic of mammalian SSC, abundant SER could be shown in the Y-organ, where it is unevenly distributed. The amount of SER seems to vary in correlation with the secretion of moulting hormone during the moult cycle. Most Y-organ cells contain a great number of mitochondria of the tubular type, another important characteristic of mammalian SSC. The ultrastructure of the mandibular organ of C. maenas differs considerably from that of the Y-organ. Some SER was found, mitochondria of unusual shape and size were conspicuous. No definite conclusion as to the function of the mandibular organ is yet to be drawn.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233610
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Somatostatin-binding sites on rat telencephalic astrocytes (1990)
    Springer
    Cell & tissue research 262 (1990), S. 431-443 
    Details
    ISSN: 1432-0878
    Keywords: Astrocytes ; Telencephalon ; Receptors, membrane ; Somatostatin (SRIF) ; Rat (Han: WIST)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using a somatostatin-gold conjugate as ligand, high-affinity binding sites for this neuropeptide were demonstrated at three levels: (i) cultured astrocytes from rat cortex, (ii) hippocampal slice cultures, and (iii) frozen tissue sections of rat telencephalon. The conjugate proved as active as the native peptide in competing for the binding sites. Light-microscopic visualization of bound ligand was achieved by silver intensification of the colloidal gold. This method is faster and yields superior resolution compared with autoradiography. Cultured astrocytes from cortex and hippocampus could be labeled by the ligand. At the light- and electron-microscopic level, astrocytes could be double-labeled by the somatostatin-gold conjugate and immunostaining for glial fibrillary acidic protein (GFAP). In hippocampal slice cultures, the conjugate did not penetrate into the neuropil because of a covering glial layer. However, a portion of this completely GFAP-positive covering glia reacted with the somatostatin ligand. In frozen brain sections, apart from delicate punctate structures, two types of labeled glia cells were seen: single stellate astrocytes and perivascular glia cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305240
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    Paper (German National Licenses)
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