ISSN:
1573-5044
Keywords:
anti-tumor activity
;
Camptotheca acuminata
;
camptothecin
;
plant cell culture
;
secondary metabolism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Camptotheca acuminata plants contain camptothecin which is a secondary metabolite with strong anti-tumor activity. The induction of callus and cell suspension cultures from Camptotheca acuminata stem parts on different media is described. Growth in the original media and in media with different salts and/or vitamins was followed by measuring several growth parameters. Camptothecin was detected and identified by means of TLC, HPLC and GC-MS. The production of camptothecin in cell suspension cultures was followed and compared to the original plant material. Results indicate that high density cultures could be easily established, producing ca 1 mg l-1 of camptothecin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00039910
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