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  • Bryde whale  (1)
  • Pisum sativum  (1)
  • TetR  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The use of a multiple antigen peptide to detect a minichromosome maintenance protein in pea (Pisum sativum) (1998)
    Springer
    Experimental biology online 3 (1998), S. 1-5 
    Details
    ISSN: 1430-3418
    Keywords: DNA polymerase ; Multiple antigen peptide ; MCM protein ; Pea ; Pisum sativum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 13-residue oligopeptide corresponding to a conserved region of the MCM family of proteins was synthesised as a multiple antigen peptide in which eight copies of the peptide were conjugated to an oligo-lysine core. The multiple antigen peptide was used for raising antibodies. Western blots of the polypeptides present in the DNA polymerase–primase complex from pea (Pisum sativum L.) were challenged with the antibodies which, even at a dilution of 1:5000, clearly revealed a polypeptide of approximately 62 kDa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00898-998-0007-1
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Codon optimization, genetic insulation, and an rtTA reporter improve performance of the tetracycline switch (1999)
    Springer
    Transgenic research 8 (1999), S. 371-382 
    Details
    ISSN: 1573-9368
    Keywords: codon bias ; IRES ; MAR ; rtTA ; TetR ; tetR ; transgenic ; transgene switch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this work was to further develop a tetracycline repressor (TetR) protein system that allows control of transgene expression. First, to circumvent the need for a binary approach, a single plasmid design was constructed and tested in tissue culture. To indirectly assay integrations that express the synthetic transcription factor (rtTA), a bicistronic gene was built which included an internal ribosome entry site (IRES) and a green fluorescent protein coding region (GFP) on the same expression cassette as the coding region of rtTA (pTetGREEN). This construct did not produce fluorescent colonies when stably integrated and provided minimal expression of GFP in the face of adequate expression of rtTA. The coding region for TetR was then altered by introducing 156 silent point mutations to simulate mammalian genes. Replacement of wild‐type TetR gene (tetR) in pTetGREEN with 'mammalianized' provided GFP expression. Adjustment of codon usage in the tetR region of rtTA nearly doubled the expression level of functional rtTA. To increase the number of rtTA expressing lines, the chicken egg‐white lysozyme matrix attachment region (MAR) was introduced into the single plasmid design just upstream of the tetracycline operators (tetO). Inclusion of the MAR doubled the number of colonies that expressed rtTA (44 vs 88). With the modifications described here, the number of lines that express rtTA and provide induction from a single plasmid design can be increased by the inclusion of a MAR and the level of rtTA expression can be further increased by adjusting the base composition of the TetR coding region. The MAR also insulates the inducible gene from the promoter driving rtTA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008952302539
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    Paper (German National Licenses)
    Fulltext
  • 3
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    Observations and radio tagging of Balaenoptera edeni near Puerto La Cruz, Venezuela (2018)
    Woods Hole Oceanographic Institution
    Details
    Publication Date: 2022-05-26
    Description: The 23 October to 13 November 1979 Venezuelan radio tagging and tracking experiments on whales (Balaenoptera edeni, Fig. 1) provided essential field tests of the new modifications to the WHOI radio whale tag (see list of tag reports), and the chance to try it on a new species. We found that we could approach and tag these whales from a slow (4 to 6 kt) vessel. Good radio tracking with automatic direction finding equipment was possible within 12 to 20 km, with longer ranges probable. In addition, the radio tags provided new information about the behavior of these whales.
    Description: Prepared for the Office of Naval Research under Contract N00014-79-C-OO71; NR 083-004.
    Keywords: Animal radio tracking ; Whales ; Bryde whale
    Repository Name: Woods Hole Open Access Server
    Type: Technical Report
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    PAPER (OA)
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