ISSN:
0170-2041
Schlagwort(e):
β-Galactosyltransferase
;
Glycoproteins, N-linked
;
Branch specificity
;
Spacer-modified oligosaccharides
;
Enzymes
;
Carbohydrates
;
Chemistry
;
Organic Chemistry
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
Differentiation of the Branch-Specific Enzymic Galactosylation by Nuclear-Magnetic Resonance SpectroscopyA spacer-modified biantennary trisaccharide glycoside (2) carrying two GlcNAc end groups was galactosylated by UDP-Gal and β-(1→4)-D-galactosyltransferase to yield compounds 3a, 3b and 4. The non-separable monogalactosylated compounds 3a and 3b were investigated by NMR spectroscopy (COSY, COLOC, RELAY) to determine a potential branch-specific transgalactosylation found for native acceptor-oligosaccharides (1). Since the “3-branch” is galactosylated at the same rate as the “6-branch” the presence not only of the center-mannosyl unit but also of the flanking mannosyl residues replaced by spacers in compound 2 are necessary for site specificity.
Zusätzliches Material:
2 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jlac.199419940104
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