ISSN:
1040-0397
Keywords:
Glucose
;
Biosensor
;
Microelectrode
;
Platinization
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Miniaturized glucose biosensors were constructed by all-chemical means from disk-shaped carbon ultramicroelectrodes (UMEs) (diameter = 8 μm). These ultramicrobiosensors (UMBs) are capable of direct measurement of glucose in the presence of interfering species. Carbon UMEs were partially platinized by the electrochemical reduction of a platinum (IV) complex. Platinization of the carbon UMEs gave an increased current response to hydrogen peroxide and allowed the modified electrode to be operated at a lower oxidative potential. Glucose oxidase (E.C. 1.1.3.4.) was immobilized either by glutaraldehyde cross-linking or by direct covalent attachment to the electrode surface using carbodiimide. Response time (100% response) was 15-45 seconds. Response was linear over the range of 3-7 mM glucose. An electropolymerized film of poly(1,3-diaminobenzene) was used to prevent interfering species.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140050506
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